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Acorn barnacle Megabalanus rosa lectin (BRA-3): cDNA cloning, gene structure and seasonal changes of mRNA and protein levels

1993; Elsevier BV; Volume: 128; Issue: 2 Linguagem: Inglês

10.1016/0378-1119(93)90570-s

1879-0038

Nobuhiko Takamatsu, Takeda Tadashi, Miho Kojima, Masayuki Heishi, Koji Muramoto, Hisao Kamiya, Tadayoshi Shiba,

Neurobiology and Insect Physiology Research

We have isolated cDNA clones coding for a lectin (BRA-3) from the acorn barnacle, Megabalanus rosa. Sequence comparison of the cDNA clones has revealed polymorphism in the BRA-3 mRNA, which results from single-nucleotide (nt) differences at three positions. All three differences are within the coding region and cause conservative amino acid (aa) changes. The BRA-3 gene is composed of four exons, and the three single-nt differences are located on different exons. In addition, the BRA-3 mRNA and BRA-3 protein levels increased during early summer in a similar fashion, indicating that BRA-3 production is regulated mainly at the level of transcription.