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Grazing-Exit and Micro X-ray Fluorescence Analyses for Chemical Microchips

2005; Springer Nature; Volume: 21; Issue: 7 Linguagem: Inglês

10.2116/analsci.21.799

1348-2246

Kouichi Tsuji, T. Emoto, Yosuke Nishida, Eiichiro Tamaki, Yoshikuni Kikutani, Akihide Hibara, Takehiko Kitamori,

Mass Spectrometry Techniques and Applications

Grazing-exit x-ray fluorescence (GE-XRF) and micro x-ray fluorescence (micro-XRF) methods were applied to chemical microchips as a detection method. Since an energy-dispersive x-ray detector was used, the simultaneous detection of multiple elements was possible. An analyzing region was especially designed on the microchip so that a sample solution could be dried and concentrated in a suitable area corresponding to the size of the primary x-ray beam. Finally, it was confirmed that both analytical methods could be combined well for use with a microchip. In GE-XRF, the background intensity in the XRF spectrum was reduced at grazing-exit angles. In addition, a good relationship between the x-ray fluorescence intensities and the concentrations of standard solutions that were introduced into the microchip was obtained. This indicates that the GE-XRF method is feasible for trace elemental analysis in chemical microchip systems. In micro-XRF, an attempt was made to concentrate and dry the analyte within a small analyzing region. The preliminary results indicated that the micro-XRF method could be applied for the analysis of microchips.