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Mooreia alkaloidigena gen. nov., sp. nov. and Catalinimonas alkaloidigena gen. nov., sp. nov., alkaloid-producing marine bacteria in the proposed families Mooreiaceae fam. nov. and Catalimonadaceae fam. nov. in the phylum Bacteroidetes

2012; Microbiology Society; Volume: 63; Issue: Pt_4 Linguagem: Inglês

10.1099/ijs.0.043752-0

1466-5034

Eun Ju Choi, Deanna S. Beatty, Lauren A. Paul, William Fenical, Paul R. Jensen,

Microbial Applications in Construction Materials

Bacterial strains CNX-216 T and CNU-914 T were isolated from marine sediment samples collected from Palmyra Atoll and off Catalina Island, respectively. Both strains were Gram-negative and aerobic and produce deep-orange to pink colonies and alkaloid secondary metabolites. Cells of strain CNX-216 T were short, non-motile rods, whereas cells of strain CNU-914 T were short, curved rods with gliding motility. The DNA G+C contents of CNX-216 T and CNU-914 T were respectively 57.7 and 44.4 mol%. Strains CNX-216 T and CNU-914 T contained MK-7 as the predominant menaquinone and iso-C 15 : 0 and C 16 : 1 ω5 c as the major fatty acids. Phylogenetic analyses revealed that both strains belong to the order Cytophagales in the phylum Bacteroidetes . Strain CNX-216 T exhibited low 16S rRNA gene sequence identity (87.1 %) to the nearest type strain, Cesiribacter roseus 311 T , and formed a well-supported lineage that is outside all currently described families in the order Cytophagales . Strain CNU-914 T shared 97.6 % 16S rRNA gene sequence identity with ‘ Porifericola rhodea ’ N5EA6-3A2B and, together with ‘ Tunicatimonas pelagia ’ N5DB8-4 and four uncharacterized marine bacteria isolated as part of this study, formed a lineage that is clearly distinguished from other families in the order Cytophagales . Based on our polyphasic taxonomic characterization, we propose that strains CNX-216 T and CNU-914 T represent novel genera and species, for which we propose the names Mooreia alkaloidigena gen. nov., sp. nov. (type strain CNX-216 T = DSM 25187 T = KCCM 90102 T ) and Catalinimonas alkaloidigena gen. nov., sp. nov. (type strain CNU-914 T = DSM 25186 T = KCCM 90101 T ) within the new families Mooreiaceae fam. nov. and Catalimonadaceae fam. nov.