Qualitative and Quantitative Expression Profile of Muscarinic Receptors in Human Urothelium and Detrusor
2006; Lippincott Williams & Wilkins; Volume: 176; Issue: 4 Linguagem: Inglês
10.1016/j.juro.2006.06.088
ISSN1527-3792
AutoresShachi Tyagi, Pradeep Tyagi, Suzy Van-le, Naoki Yoshimura, Michael B. Chancellor, Fernando de Miguel,
Tópico(s)Urinary Tract Infections Management
ResumoNo AccessJournal of UrologyInvestigative urology1 Oct 2006Qualitative and Quantitative Expression Profile of Muscarinic Receptors in Human Urothelium and Detrusoris corrected byErratais companion of99mTechnetium-Mercaptoacetyltriglycine Scintigraphy With Full Bladder in Patients With Severe Bladder Dysfunction Shachi Tyagi, Pradeep Tyagi, Suzy Van-le, Naoki Yoshimura, Michael B. Chancellor, and Fernando de Miguel Shachi TyagiShachi Tyagi , Pradeep TyagiPradeep Tyagi , Suzy Van-leSuzy Van-le , Naoki YoshimuraNaoki Yoshimura , Michael B. ChancellorMichael B. Chancellor , and Fernando de MiguelFernando de Miguel View All Author Informationhttps://doi.org/10.1016/j.juro.2006.06.088AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We compared the complete spectrum of receptor subtypes expressed by human detrusor and its primary culture with the expression profile in a human urothelium immortalized cell line, and in fresh urothelium tissue and its primary cell culture. Materials and Methods: The levels of mRNA expressed for receptor subtypes M1 through M5 were determined with reverse transcriptase-polymerase chain reaction and quantitative polymerase chain reaction in total RNA extracted individually from different human bladder specimens, including fresh tissue of human urothelium and detrusor, and their respective primary cultures, as well as from the UROtsa cell line. Results: All 5 muscarinic receptors were detected in fresh human bladder tissue by reverse transcriptase-polymerase chain reaction RNA. The same was true in separated urothelium and detrusor tissue except for the lack of the M5 receptor transcript. Receptor subtype mRNA expression in the UROtsa cell line paralleled expression in fresh human bladder. Quantitative polymerase chain reaction data further corroborated these results and showed comparable mRNA expression for M2 and M3 in primary detrusor cultures. Primary cultures also had a decreased copy number of receptor genes than native tissue. The decrease was even more pronounced in primary urothelium culture and the UROtsa cell line in the presence of high calcium. M2 and M3 receptors were also detected in urothelium and detrusor by immunoreactivity. Conclusions: We identified all 5 existing muscarinic receptor subtypes in detrusor and urothelium, and transcripts levels of M2 and M3 were comparable in detrusor. These results support an alternative site of action in urothelium for anti-muscarinic drugs. Urothelial receptors should be considered in the design of future drugs for overactive bladder. Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania© 2006 by American Urological AssociationFiguresReferencesRelatedDetails References 1 : Muscarinic receptor subtypes. Annu Rev Pharmacol Toxicol1990; 30: 633. Google Scholar 2 : Autoradiographic localization of muscarinic receptors in diabetic rat bladder. Nippon Hinyokika Gakkai Zasshi1997; 88: 858. Google Scholar 3 : Urothelium-derived inhibitory factor(s) influences on detrusor muscle contractility in vitro. Br J Pharmacol2000; 129: 416. Crossref, Medline, Google Scholar 4 : Muscarinic acetylcholine receptor subtypes mediating urinary bladder contractility and coupling to GTP binding proteins. J Pharmacol Exp Ther1995; 273: 959. 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Link, Google Scholar Cited byKashyap M, Kawamorita N, Tyagi V, Sugino Y, Chancellor M, Yoshimura N and Tyagi P (2013) Down-Regulation of Nerve Growth Factor Expression in the Bladder by Antisense Oligonucleotides as New Treatment for Overactive BladderJournal of Urology, VOL. 190, NO. 2, (757-764), Online publication date: 1-Aug-2013.Datta S, Roosen A, Pullen A, Popat R, Rosenbaum T, Elneil S, Dasgupta P, Fowler C and Apostolidis A (2010) Immunohistochemical Expression of Muscarinic Receptors in the Urothelium and Suburothelium of Neurogenic and Idiopathic Overactive Human Bladders, and Changes With Botulinum Neurotoxin AdministrationJournal of Urology, VOL. 184, NO. 6, (2578-2585), Online publication date: 1-Dec-2010.Related articlesJournal of Urology9 Nov 2018ErrataJournal of Urology9 Nov 201899mTechnetium-Mercaptoacetyltriglycine Scintigraphy With Full Bladder in Patients With Severe Bladder Dysfunction" class="teaser__item__title">99mTechnetium-Mercaptoacetyltriglycine Scintigraphy With Full Bladder in Patients With Severe Bladder Dysfunction Volume 176Issue 4October 2006Page: 1673-1678 Advertisement Copyright & Permissions© 2006 by American Urological AssociationKeywordsmuscarinicurotheliumurinary incontinencereceptorsbladdergene expressionAcknowledgmentsDoctor Sens, Department of Pathology, University of North Dakota provided the UROtsa cell line.MetricsAuthor Information Shachi Tyagi More articles by this author … View all authors Expand All Advertisement PDF downloadLoading ...
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