Influence of birefringence on polarization resolved nonlinear microscopy and collagen SHG structural imaging
2010; Optica Publishing Group; Volume: 18; Issue: 14 Linguagem: Inglês
10.1364/oe.18.014859
ISSN1094-4087
AutoresSophie Brasselet, Dora Aït-Belkacem, Alicja Gąsecka, Fabiana Munhoz, Sophie Brustlein, Sophie Brasselet,
Tópico(s)Optical Coherence Tomography Applications
ResumoWe analyze the influence of the anisotropy of molecular and biological samples on polarization resolved nonlinear microscopy imaging. We show in particular the detrimental influence of birefringence on Second Harmonic Generation (SHG) and Two-Photon Excited Fluorescence (TPEF) polarization resolved microscopy imaging, which, if not accounted for, can lead to an erroneous determination of the sample properties and thus to a misinterpretation of the read-out information. We propose a method to measure this birefringence and account for this effect in nonlinear polarization resolved experiments.
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