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Simultaneous determination of malachite green and its metabolite leucomalachite green in eel plasma using post-column oxidation

1995; Elsevier BV; Volume: 669; Issue: 2 Linguagem: Inglês

10.1016/0378-4347(95)00112-v

1872-812X

C.A.J. Hajee, N. Haagsma,

Biochemical Analysis and Sensing Techniques

A rapid HPLC method with solid-phase extraction (SPE) clean-up for malachite green (MG) and leucomalachite green (LMG) in eel plasma was developed. MG and LMG were extracted with a buffered methanolic solution. The extract was subjected to aromatic sulphonic acid SPE. MG and LMG were eluted from the SPE column with methanol after a treatment with ammonia gas. The reconstituted eluate was analyzed on a Chromspher B column with acetonitrile-ion-pair buffer (ph 4.0) (6:4, v/v) as the mobile phase and detection at 610 nm after post column oxidation with PbO2. The average recoveries for MG and LMG over the linear range of applicability (20–2500 ng/ml) were 82±1% and 83±1%, respectively. The limits of quantification were 5.0 μg/1 for MG and 0.9 μ/1 for LMG.