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Development of a simple, rapid and validated square wave voltametric method for determination of promethazine in raw material and pharmaceutical formulation using DNA modified multiwall carbon nanotube paste electrode

2012; Elsevier BV; Volume: 177; Linguagem: Inglês

10.1016/j.snb.2012.11.005

1873-3077

João Paulo Marco, Keyller Bastos Borges, César Ricardo Teixeira Tarley, Emerson Schwingel Ribeiro, Arnaldo César Pereira,

Electrochemical Analysis and Applications

A simple voltammetric method based on a composite biosensor MWCN/SiO2/Al2O3/Nb2O5/DNA (MWCN/SiAlNb/DNA) was developed and validated for the determination of promethazine (PHZ) in raw material and pharmaceutical formulation. The best results were obtained by using square wave voltammetry under measurements at 0.30 mol L−1 phosphate buffer pH 7.0 and DNA immobilization onto SiAlNb surface using 0.8 mg mL−1 concentration. Validation parameters such as: selectivity, linearity, precision, accuracy, and stability presented results within the acceptable range. The sensitivity of sensor was found to be 0.210 μA μmol−1 L. Therefore, the developed method was successfully applied for the PHZ determination in real samples of pharmaceutical formulations and can be used for routine quality control analysis of bulk pharmaceutical and pharmaceutical formulations containing PHZ.