Water-Soluble Conjugate of Double-Stranded DNA and Poly( N -isopropylacrylamide) for One-Pot Affinity Precipitation Separation of DNA-Binding Proteins
1998; American Chemical Society; Volume: 9; Issue: 6 Linguagem: Inglês
10.1021/bc980019f
ISSN1520-4812
AutoresDaisuke Umeno, Masafumi Kawasaki, Mizuo Maeda,
Tópico(s)RNA Interference and Gene Delivery
ResumoPoly(N-isopropylacrylamide) having a terminal psoralen group was synthesized and covalently bound to plasmid pBR 322 DNA through a photochemical reaction. The resulting conjugate exhibited temperature-responsive precipitation due to the aggregation of poly(N-isopropylacrylamide) chains when heated above 31 °C. This system was used for the one-pot separation of restriction endonuclease EcoRI.
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