5-Lipoxygenase from rat PMN lysate

1986; Elsevier BV; Volume: 31; Issue: 3 Linguagem: Inglês

10.1016/0090-6980(86)90117-6

ISSN

1878-416X

Autores

Mark T. Skoog, James S. Nichols, Jeffrey S. Wiseman,

Tópico(s)

Peroxisome Proliferator-Activated Receptors

Resumo

The products of arachidonic acid metabolism in the 15,000xg supernatant of sonicated rat PMN are described. Only products derived from 5-lipoxygenase are observed. These products are 5-HETE and products derived from hydrolysis of LTA4, particularly LTB4. Some minor products derived from decomposition of 5-HPETE are also observed. The dependence of the activity of 5-lipoxygenase on enzyme and on substrate concentrations is presented and discussed in terms of a kinetic model that includes enzyme inactivation during turnover and substrate inhibition. The 5-lipoxygenase activity is stimulated by Ca++ and ATP.

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