Portal de Periódicos da CAPES

Semisynthesis of Fluorescent Metabolite Sensors on Cell Surfaces

2011; American Chemical Society; Volume: 133; Issue: 40 Linguagem: Inglês

10.1021/ja206915m

1943-2984

Matthias A. Brun, Rudolf Griss, Luc Reymond, Kui‐Thong Tan, Joachim Piguet, Ruud J. R. W. Peters, Horst Vogel, Kai Johnsson,

Click Chemistry and Applications

Progress in understanding signal transduction and metabolic pathways is hampered by a shortage of suitable sensors for tracking metabolites, second messengers, and neurotransmitters in living cells. Here we introduce a class of rationally designed semisynthetic fluorescent sensor proteins, called Snifits, for measuring metabolite concentrations on the cell surface of mammalian cells. Functional Snifits are assembled on living cells through two selective chemical labeling reactions of a genetically encoded protein scaffold. Our best Snifit displayed fluorescence intensity ratio changes on living cells significantly higher than any previously reported cell-surface-targeted fluorescent sensor protein. This work establishes a generally applicable and rational strategy for the generation of cell-surface-targeted fluorescent sensor proteins for metabolites of interest.