Portal de Periódicos da CAPES

CE‐MS in biomarker discovery, validation, and clinical application

2010; Wiley; Volume: 5; Issue: 1-2 Linguagem: Inglês

10.1002/prca.201000058

1862-8354

Harald Mischak, Joost P. Schanstra,

Biosensors and Analytical Detection

CE coupled MS (CE-MS) has become an increasingly employed technology in proteome analysis with focus on the identification of biomarker peptides in clinical proteomics. In this review, we will cover technical aspects of CE-MS coupling and highlight the improvements made in the last few years. We examine CE-MS from an application point of view, and evaluate its merits and vices for biomarker discovery and clinical applications. We discuss the principal theoretical and practical obstacles encountered when employing CE-MS (and most other proteomic technologies) for the analysis of body fluids for biomarker discovery. We will present several examples of a successful application of CE-MS for biomarker discovery, implications for disease diagnosis, prognosis, and therapy evaluation, and will discuss current challenges and possible future improvements.