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Preparative isolation of cyclolanostane‐type saponins from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by TLC‐MS/MS guided high‐speed counter‐current chromatography

2006; Wiley; Volume: 30; Issue: 1 Linguagem: Inglês

10.1002/jssc.200600264

1615-9314

Quan‐Bin Han, Jing Zheng Song, Chun Feng Qiao, Lina Wong, Hong‐Xi Xu,

Natural product bioactivities and synthesis

Two cyclolanostane-type saponins, astragalosides I and II, were first identified by TLC-MS/MS in the ethyl acetate extract of the roots of Astragalus membranaceus Bge var. mongholicus (Bge.) Hsiao without chemical reference substances. They were then isolated by high-speed counter-current chromatography with a two-step two-phase solvent system of ethyl acetate-2-propanol-water (5:1:5, 50:1:50, v/v/v). The quantities of astragalosides I and II isolated from 1 g of the crude extract were 30.2 mg and 16.5 mg, respectively. Their purities were found to be over 95% by HPLC-ELSD analysis. Their chemical structures were confirmed by 1H and 13C nuclear magnetic resonance analysis.