Mouse Coronary Angiograph Using Synchrotron Radiation Microangiography
2002; Lippincott Williams & Wilkins; Volume: 105; Issue: 2 Linguagem: Inglês
10.1161/hc0202.100423
ISSN1524-4539
AutoresTomoya Yamashita, Seinosuke Kawashima, Masanori Ozaki, Masayuki Namiki, Tetsuaki Hirase, Nobutaka Inoue, Ken–ichi Hirata, Keiji Umetani, Kazuro Sugimura, Mitsuhiro Yokoyama,
Tópico(s)Cardiomyopathy and Myosin Studies
ResumoHomeCirculationVol. 105, No. 2Mouse Coronary Angiograph Using Synchrotron Radiation Microangiography Free AccessOtherPDF/EPUBAboutView PDFView EPUBSections ToolsAdd to favoritesDownload citationsTrack citationsPermissionsDownload Articles + Supplements ShareShare onFacebookTwitterLinked InMendeleyReddit Jump toSupplemental MaterialFree AccessOtherPDF/EPUBMouse Coronary Angiograph Using Synchrotron Radiation Microangiography Tomoya Yamashita, Seinosuke Kawashima, Masanori Ozaki, Masayuki Namiki, Tetsuaki Hirase, Nobutaka Inoue, Ken-ichi Hirata, Keiji Umetani, Kazuro Sugimura and Mitsuhiro Yokoyama Tomoya YamashitaTomoya Yamashita From the Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine (T.Y., S.K., M.O., M.N., T.H., N.I., K.H., M.Y.), and the Department of Radiology (K.S.), Kobe University Graduate School of Medicine, Kobe, Japan, and the Japan Synchrotron Radiation Research Institute, Harima, Japan (K.U.). , Seinosuke KawashimaSeinosuke Kawashima From the Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine (T.Y., S.K., M.O., M.N., T.H., N.I., K.H., M.Y.), and the Department of Radiology (K.S.), Kobe University Graduate School of Medicine, Kobe, Japan, and the Japan Synchrotron Radiation Research Institute, Harima, Japan (K.U.). , Masanori OzakiMasanori Ozaki From the Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine (T.Y., S.K., M.O., M.N., T.H., N.I., K.H., M.Y.), and the Department of Radiology (K.S.), Kobe University Graduate School of Medicine, Kobe, Japan, and the Japan Synchrotron Radiation Research Institute, Harima, Japan (K.U.). , Masayuki NamikiMasayuki Namiki From the Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine (T.Y., S.K., M.O., M.N., T.H., N.I., K.H., M.Y.), and the Department of Radiology (K.S.), Kobe University Graduate School of Medicine, Kobe, Japan, and the Japan Synchrotron Radiation Research Institute, Harima, Japan (K.U.). , Tetsuaki HiraseTetsuaki Hirase From the Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine (T.Y., S.K., M.O., M.N., T.H., N.I., K.H., M.Y.), and the Department of Radiology (K.S.), Kobe University Graduate School of Medicine, Kobe, Japan, and the Japan Synchrotron Radiation Research Institute, Harima, Japan (K.U.). , Nobutaka InoueNobutaka Inoue From the Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine (T.Y., S.K., M.O., M.N., T.H., N.I., K.H., M.Y.), and the Department of Radiology (K.S.), Kobe University Graduate School of Medicine, Kobe, Japan, and the Japan Synchrotron Radiation Research Institute, Harima, Japan (K.U.). , Ken-ichi HirataKen-ichi Hirata From the Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine (T.Y., S.K., M.O., M.N., T.H., N.I., K.H., M.Y.), and the Department of Radiology (K.S.), Kobe University Graduate School of Medicine, Kobe, Japan, and the Japan Synchrotron Radiation Research Institute, Harima, Japan (K.U.). , Keiji UmetaniKeiji Umetani From the Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine (T.Y., S.K., M.O., M.N., T.H., N.I., K.H., M.Y.), and the Department of Radiology (K.S.), Kobe University Graduate School of Medicine, Kobe, Japan, and the Japan Synchrotron Radiation Research Institute, Harima, Japan (K.U.). , Kazuro SugimuraKazuro Sugimura From the Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine (T.Y., S.K., M.O., M.N., T.H., N.I., K.H., M.Y.), and the Department of Radiology (K.S.), Kobe University Graduate School of Medicine, Kobe, Japan, and the Japan Synchrotron Radiation Research Institute, Harima, Japan (K.U.). and Mitsuhiro YokoyamaMitsuhiro Yokoyama From the Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine (T.Y., S.K., M.O., M.N., T.H., N.I., K.H., M.Y.), and the Department of Radiology (K.S.), Kobe University Graduate School of Medicine, Kobe, Japan, and the Japan Synchrotron Radiation Research Institute, Harima, Japan (K.U.). Originally published15 Jan 2002https://doi.org/10.1161/hc0202.100423Circulation. 2002;105:e3–e4Genetically engineered mice provide enormous potential to study the pathogenesis and treatment of atherosclerosis. However, most experimental designs to investigate mouse coronary atherosclerosis are limited to in vitro and ex vivo examinations. Technological advances in microangiography have facilitated the detailed study of microvessels ex vivo and in vivo; the development of synchrotron radiation (SR) microangiography has enabled us to study mouse coronary arteries in moving hearts. For high-resolution, real-time imaging (10×5 mm, 30 frames/second), a monochromatized x-ray obtained from the third generation SR facility (SPring-8, Japan) and a new x-ray SATICON camera (Hamamatsu Photonics) were used. Digital microangiography with 7-μm pixel sizes was performed.First, we performed mouse coronary angiography using ex vivo working hearts under the Langendorff perfusion technique and detected coronary atherosclerotic lesions in 5-month-old, cholesterol-fed, apolipoprotein E knockout mice. We also performed in vivo mouse coronary angiography. C57BL/6 mice (25 to 28 g) were anesthetized with pentobarbital, and a SP-8 polyethylene tube (0.5 mm in diameter, Natsume Manufactory) was introduced into the aortic valve through the right carotid artery to infuse iodine contrast agents. As shown in the Figure and the accompanying cine images (can be found Online at http://www.circulationaha.org), use of this system provided us with serial examinations of coronary arteries in situ in genetically engineered mice. Consequently, this system is a powerful tool to study the pathophysiology of coronary artery disease. Download figureDownload PowerPointTop, Coronary angiograph from an apolipoprotein E knockout mouse fed a high-cholesterol diet for 5 months. The heart was removed and perfused with 95% O2-saturated Krebs-Henseleit solution (at 37°C with 60 mm Hg constant pressure) using a Langendorff apparatus. Pacing was used to keep the heart rate at >60 beats/min, and iodine contrast agents (40% iodine; 0.1 mL/s for 2 s) were injected through a needle (22G) attached to the ascending aorta of the mouse heart. At the proximal end of the left coronary artery, a severe stenotic lesion was detected (arrow). Bar=500 μm. Top right, Histological analysis of the stenotic lesion. Bar=100 μm. Bottom, In vivo coronary angiograph of an anesthetized C57BL/6 mouse. Iodine contrast agents (40% iodine; 0.1 mL/s for 2 s) were injected through a tube inserted from the right carotid artery. The diameter of a copper wire (arrowhead) is 100 μm. Cine images of the bottom panel of this figure can be seen at http://www.circulatiohaha.org.Movie versions of the Figure can be found in a Data Supplement at http://www.circulationaha.orgFootnotesCorrespondence to Seinosuke Kawashima, MD, PhD, Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. E-mail [email protected] eLetters(0)eLetters should relate to an article recently published in the journal and are not a forum for providing unpublished data. Comments are reviewed for appropriate use of tone and language. Comments are not peer-reviewed. Acceptable comments are posted to the journal website only. Comments are not published in an issue and are not indexed in PubMed. Comments should be no longer than 500 words and will only be posted online. References are limited to 10. Authors of the article cited in the comment will be invited to reply, as appropriate.Comments and feedback on AHA/ASA Scientific Statements and Guidelines should be directed to the AHA/ASA Manuscript Oversight Committee via its Correspondence page.Sign In to Submit a Response to This Article Previous Back to top Next FiguresReferencesRelatedDetailsCited By Patzelt M, Mrzilkova J, Dudak J, Krejci F, Zemlicka J, Karch J, Musil V, Rosina J, Sykora V, Horehledova B and Zach P (2019) Ethanol fixation method for heart and lung imaging in micro-CT, Japanese Journal of Radiology, 10.1007/s11604-019-00830-6, 37:6, (500-510), Online publication date: 1-Jun-2019. 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