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DNA or cell kinetics flow cytometry analysis of 33 small gastrointestinal cancers treated by photodynamic therapy

1994; Wiley; Volume: 73; Issue: 6 Linguagem: Inglês

10.1002/1097-0142(19940315)73

1097-0142

Marie-Thérèse Foultier, Véronique Vonarx-Coinsman, Léonor Xavier de Brito, Laurent Morlet, Nelly Robillard, Thierry Patrice,

Cancer Research and Treatments

CancerVolume 73, Issue 6 p. 1595-1607 Original ArticleFree Access DNA or cell kinetics flow cytometry analysis of 33 small gastrointestinal cancers treated by photodynamic therapy Marie-Thérèse Foultier Ph.D., Marie-Thérèse Foultier Ph.D. Physiologie, 6eme étage, Faculté de Pharmacie, Hǒpital LaennecSearch for more papers by this authorVéronique Vonarx-Coinsman Ph.D., Véronique Vonarx-Coinsman Ph.D. Physiologie, 6eme étage, Faculté de Pharmacie, Hǒpital LaennecSearch for more papers by this authorLeonor Xavier De Brito M.D., Leonor Xavier De Brito M.D. Département Laser, Clinique Gastroenterologique, Hǒpital LaennecSearch for more papers by this authorLaurent Morlet, Laurent Morlet Physiologie, 6eme étage, Faculté de Pharmacie, Hǒpital LaennecSearch for more papers by this authorNelly Robillard Ph.D., Nelly Robillard Ph.D. Cytometry Unit, Hǒtel-Dieu, 44035 Nantes Cedex, FranceSearch for more papers by this authorThierry Patrice M.D., Ph.D., Corresponding Author Thierry Patrice M.D., Ph.D. Département Laser, Clinique Gastroenterologique, Hǒpital LaennecPhysiologie, 6eme étage Faculté de Pharmacie, 44035 Nantes Cedex, France===Search for more papers by this author Marie-Thérèse Foultier Ph.D., Marie-Thérèse Foultier Ph.D. Physiologie, 6eme étage, Faculté de Pharmacie, Hǒpital LaennecSearch for more papers by this authorVéronique Vonarx-Coinsman Ph.D., Véronique Vonarx-Coinsman Ph.D. Physiologie, 6eme étage, Faculté de Pharmacie, Hǒpital LaennecSearch for more papers by this authorLeonor Xavier De Brito M.D., Leonor Xavier De Brito M.D. Département Laser, Clinique Gastroenterologique, Hǒpital LaennecSearch for more papers by this authorLaurent Morlet, Laurent Morlet Physiologie, 6eme étage, Faculté de Pharmacie, Hǒpital LaennecSearch for more papers by this authorNelly Robillard Ph.D., Nelly Robillard Ph.D. Cytometry Unit, Hǒtel-Dieu, 44035 Nantes Cedex, FranceSearch for more papers by this authorThierry Patrice M.D., Ph.D., Corresponding Author Thierry Patrice M.D., Ph.D. Département Laser, Clinique Gastroenterologique, Hǒpital LaennecPhysiologie, 6eme étage Faculté de Pharmacie, 44035 Nantes Cedex, France===Search for more papers by this author First published: 15 March 1994 https://doi.org/10.1002/1097-0142(19940315)73:6 3.0.CO;2-7Citations: 19AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Background. Photodynamic therapy (PDT) mediated by hematoporphyrin derivative (HPD) is a new treatment for cancers of small volume undergoing Phase II or III clinical trials in various medical fields. However, there is a lack of prognostic criteria of efficacy as in other cancer treatment. Methods. Cell DNA content or cell kinetics throughout the cell cycle were analyzed by flow cytometry and propidium iodide staining before and after HPD-PDT in 33 patients with Tis or T1 cancers of the gastrointestinal tract. The authors compared results in near-diploid cancers with those obtained in normal corresponding tissue. Results. Complete local tumor destruction and negative histologic findings (complete response [CR]) were observed in 17 of 33 patients during a period averaging 15.7 months. Flow cytometry DNA analysis was feasible in 32 patients. Aneuploidy, found in 15 of the 32 indicated a poor prognosis because 5 of 15 patients with aneuploid tumors were classified as having CR, compared with 12 of 17 patients with near-diploid tumors (P < 0.05). Changes in ploidy after PDT in 11 patients consisted of a reduction in the number of aneuploid peaks in 8 patients and the appearance of one aneuploid peak in 3 patients. Percentages of cells in SG2M phase in near-diploid tumors differed from those observed in control subjects for adenocarcinomas, and there was no significant decrease after HPD-PDT. There was no correlation between the decrease of SG2M cells and the response to HPD-PDT. Conclusion. Results obtained with PDT in this series of patients confirm previously published findings. Changes occurring in the ploidy of PDT-treated patients demonstrate that PDT acts directly on cancer cells in humans and not only on tumor vasculature. However, response to PDT varies from one cell population to another. The appearance of aneuploid populations after PDT suggests that destruction of sensitive cell populations allows the growth of aneuploid clones that initially are not detectable by flow cytometry. Citing Literature Volume73, Issue615 March 1994Pages 1595-1607 ReferencesRelatedInformation