A polymer-nucleotide linkage useful for the solid phase synthesis of cyclic oligodeoxyribonucleotides
1989; Elsevier BV; Volume: 45; Issue: 14 Linguagem: Inglês
10.1016/s0040-4020(01)89087-6
ISSN1464-5416
AutoresStefania Barbato, Lorenzo De Napoli, Luciano Mayol, Gennaro Piccialli, Ciro Santacroce,
Tópico(s)Carbohydrate Chemistry and Synthesis
Resumo2′-Deoxycytidine-, 2′-deoxyadenosine- and 2′-deoxyguanosine-5′-O-(4,4′-dimethoxytriphenylmethyl)-3′-O-(2-chlorophenyl-2-cyanoethyl)phosphate have been anchored to a solid support (polydimethylacrylamide), through the exocyclic amino group. 2′-Deoxythymidine-5′-O-(4,4′-dimethoxytriphenylmethyl)-3′-O-(2-chlororophenyl-2-cyanoethyl)phosphate has been attached as well, by means of the N3 and/or O4 positions of the base moiety. The resulting polymer, which permits chain elongation from either end, can be conveniently used for the solid phase synthesis of cyclic oligodeoxyribonucleotides. The preparation of a number of such molecules containing up to 10 base residues by the phosphotriester approach is described.
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