A PRIMATE MODEL OF RENAL ISCHEMIA-REPERFUSION INJURY FOR PRECLINICAL EVALUATION OF THE ANTILEUKOCYTE FUNCTION ASSOCIATED ANTIGEN 1 MONOCLONAL ANTIBODY ODULIMONAB
2001; Lippincott Williams & Wilkins; Volume: 166; Issue: 5 Linguagem: Inglês
10.1016/s0022-5347(05)65720-5
ISSN1527-3792
AutoresMarco J. da Silva, Palmina Petruzzo, Silvina Ramella Virieux, Jérôme Tiollier, Lionel Badet, Xavier Martín,
Tópico(s)Immunotherapy and Immune Responses
ResumoNo AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Nov 2001A PRIMATE MODEL OF RENAL ISCHEMIA-REPERFUSION INJURY FOR PRECLINICAL EVALUATION OF THE ANTILEUKOCYTE FUNCTION ASSOCIATED ANTIGEN 1 MONOCLONAL ANTIBODY ODULIMONAB MARCO DA SILVA, PALMINA PETRUZZO, SILVINA VIRIEUX, JEROME TIOLLIER, LIONEL BADET, and XAVIER MARTIN MARCO DA SILVAMARCO DA SILVA More articles by this author , PALMINA PETRUZZOPALMINA PETRUZZO More articles by this author , SILVINA VIRIEUXSILVINA VIRIEUX More articles by this author , JEROME TIOLLIERJEROME TIOLLIER More articles by this author , LIONEL BADETLIONEL BADET More articles by this author , and XAVIER MARTINXAVIER MARTIN More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)65720-5AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We established a primate model to investigate the effects of the antileukocyte function associated antigen 1 (CD 11a) mAb odulimomab (Imtix-Sangstad, Lyon, France) for preventing renal ischemia-reperfusion injury. Materials and Methods: We randomly divided 34 Macaca cynomolgus monkeys into groups 1 and 2, which received a renal autograft after 2 hours of cold ischemia, and groups 3 and 4, which received the autograft after 24 hours of cold ischemia. Before cold ischemia all harvested kidneys were subjected to 30 to 45 minutes of warm ischemia. Groups 1 and 3 monkeys were treated with an antileukocyte function associated antigen 1 mAb before cold ischemia and then for 3 days, while groups 2 and 4 monkeys received an IgG1 isotype control. In all groups renal function was investigated before warm ischemia and 72 hours after reperfusion. Serum creatinine and the leukocyte count were determined daily. Histological studies were done and lactoferrin was measured in the autotransplanted kidney 72 hours after reperfusion. Results: A decrease in renal function was shown after 2 hours of cold ischemia with tubular necrosis and mild cell infiltration, while after 24 hours of cold ischemia there was severe renal failure with tubular and glomerular necrosis, and leukocyte infiltration. A significant improvement in renal function and decrease in kidney lactoferrin content was evident in group 1 compared to group 2 at 72 hours, while no significant difference was noted in groups 3 and 4. No difference in histological patterns was evident in treated and untreated animals. Conclusions: This study provides evidence for the validity of this ischemia-reperfusion injury model in primates. The protective effects of antileukocyte function associated antigen 1 mAb on renal injury was not as dramatic as in rodent models but a significant improvement in renal function was observed in treated animals after 2 hours of cold ischemia. References 1 : Increased incidence of rejection in patients with delayed graft function. 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Google Scholar 20 : Involvement of endogenous interleukin-10 and tumor necrosis factor-alpha in renal ischaemia-reperfusion injury. Transplantation1999; 67: 792. Google Scholar From the Service of Urology and Transplantation, INSERM U281 and Imtix-Sangstat, Lyon, France© 2001 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 166Issue 5November 2001Page: 1915-1919 Advertisement Copyright & Permissions© 2001 by American Urological Association, Inc.Keywordskidneyischemiakidney transplantationMacaca fascicularislymphocyte function associated antigen-1MetricsAuthor Information MARCO DA SILVA More articles by this author PALMINA PETRUZZO More articles by this author SILVINA VIRIEUX More articles by this author JEROME TIOLLIER More articles by this author LIONEL BADET More articles by this author XAVIER MARTIN More articles by this author Expand All Advertisement PDF downloadLoading ...
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