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Microbial Contamination in Next Generation Sequencing: Implications for Sequence-Based Analysis of Clinical Samples

2014; Public Library of Science; Volume: 10; Issue: 11 Linguagem: Inglês

10.1371/journal.ppat.1004437

1553-7374

Michael J. Strong, Guorong Xu, Lisa A. Morici, Sandra Splinter BonDurant, Melody Baddoo, Zhen Lin, Claire Fewell, Christopher M. Taylor, Erik K. Flemington,

Enterobacteriaceae and Cronobacter Research

The high level of accuracy and sensitivity of next generation sequencing for quantifying genetic material across organismal boundaries gives it tremendous potential for pathogen discovery and diagnosis in human disease. Despite this promise, substantial bacterial contamination is routinely found in existing human-derived RNA-seq datasets that likely arises from environmental sources. This raises the need for stringent sequencing and analysis protocols for studies investigating sequence-based microbial signatures in clinical samples.