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Characterization of a New Virus from Garlic(Allium sativum L.), Garlic Mite-borne Mosaic Virus.

1996; Phytopathological Society of Japan; Volume: 62; Issue: 5 Linguagem: Inglês

10.3186/jjphytopath.62.483

1882-0484

Kazuo Yamashita, Jun‐ichi Sakai, Kaoru Hanada,

Plant Pathogenic Bacteria Studies

Flexuous, filamentous virus particles with a length of 700-800nm were detected in garlic (Allium sativum L.) plants showing mosaic symptoms. The virus was sap-transmissible from garlic plants to seven out of 25 plant species tested, inducing local lesions on Chenopodium murale and Gomphrena globosa and a systemic mosaic on garlic. This virus was transmitted by the eriophyid mite, Aceria tulipae Keifer, but not by aphids. Two polypeptides with M.W. 30kDa and 28.5kDa were detected by SDS-PAGE of the purified virus preparation. The virus exhibited no serological relationships with leek yellow stripe, onion yellow dwarf or garlic latent viruses. No cytoplasmic cylindrical inclusions were observed in infected leaf cells of garlic or C. murale. The 3'-terminal nucleotide sequence of 2518bp was determined. It contained three putative open reading frames (ORFs) for 40kDa, 28kDa (a putative coat protein, CP) and 15kDa polypeptides. The arrangement and amino acid sequences of these ORFs show close similarities with those of shallot virus X and garlic virus (GV)-A, -B, -C and -D. The putative CP of the mite-borne virus has 98% amino acid sequence homology with CP of GV-C, but only 60-67% identity with CPs of four other viruses, indicating the classification of the mite-borne virus and GV-C as the same virus. We propose the name garlic mite-borne mosaic virus (GMbMV) for the virus.