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A new single-platform method for the enumeration of CD34+ cells

2009; Elsevier BV; Volume: 11; Issue: 6 Linguagem: Inglês

10.3109/14653240903092628

1477-2566

Claude Lemarié, Guillaume Bouchet, I Sielleur, Véronique Durand, F.H. Navarro, Boris Calmels, Christian Chabannon,

Hematopoietic Stem Cell Transplantation

Abstract Guidelines for flow cytometric enumeration of CD34 + hematopoietic stem cells (HSC) recommend the use of a single-platform assay. The SCE kit has recently been commercialized by BD Biosciences. Results obtained with this newly available kit were compared with CD34 + cell enumerations obtained in parallel with already commercialized diagnostic kits; fresh peripheral blood, apheresis, cord blood (CB) and bone marrow (BM) samples, as well as thawed apheresis and CB samples, were assayed. The SCE kit produced data for CD34 + enumeration that correlate well with data produced with the older assays ( r 2 ≥0.9). Practical advantages were the ability to enumerate viable CD34 cells in all kinds of HSC products, the absence of bead pipetting (which decreases results precision) and a gating strategy complying with international recommendations. A major disadvantage was the absence of specific software for data analyses and presentation of results.