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Evaluation of an Injectable, Photopolymerizable, and Three-Dimensional Scaffold Based on Methacrylate-Endcapped Poly(D,L-Lactide-co-ɛ-Caprolactone) Combined with Autologous Mesenchymal Stem Cells in a Goat Tibial Unicortical Defect Model

2008; Mary Ann Liebert, Inc.; Volume: 15; Issue: 7 Linguagem: Inglês

10.1089/ten.tea.2008.0367

1937-335X

Geert Vertenten, Evi Lippens, Jordi Gironès, Tomasz Gorski, Heidi Declercq, Jimmy Saunders, Wim Van Den Broeck, Koen Chiers, Luc Duchateau, Etiene Schacht, Maria Cornelissen, Frank Gasthuys, Lieven Vlaminck,

Dental Implant Techniques and Outcomes

An in situ crosslinkable, biodegradable, methacrylate-endcapped poly(D,L-lactide-co-epsilon-caprolactone) in which crosslinkage is achieved by photoinitiators was developed for bone tissue regeneration. Different combinations of the polymer with bone marrow-derived mesenchymal stem cells (BMSCs) and alpha-tricalcium phosphate (alpha-TCP) were tested in a unicortical tibial defect model in eight goats. The polymers were randomly applied in one of three defects (6.0 mm diameter) using a fourth unfilled defect as control. Biocompatibility and bone-healing characteristics were evaluated by serial radiographies, histology, histomorphometry, and immunohistochemistry. The results demonstrated cell survival and proliferation in the polymer-substituted bone defects. The addition of alpha-TCP was associated with less expansion and growth of the BMSCs than other polymer composites.