A highly selective turn-on fluorescent probe for iron(ii) to visualize labile iron in living cells
2012; Royal Society of Chemistry; Volume: 4; Issue: 3 Linguagem: Inglês
10.1039/c2sc21649c
ISSN2041-6539
AutoresTasuku Hirayama, K Okuda, Hideko Nagasawa,
Tópico(s)Electrochemical sensors and biosensors
ResumoAlthough labile iron plays critical roles in diverse biological processes in living cells, the physiological and pathophysiological functions of iron have not been sufficiently explored, partially due to a lack of methods for visualizing intracellular labile iron. In this edge article, we present a novel turn-on fluorescent probe (RhoNox-1) for the selective detection of Fe2+ based on N-oxide chemistry. Spectroscopic studies combined with DFT calculations and electrochemical studies revealed that fluorescence quenching of RhoNox-1 occurred in physiological conditions, which was attributed to non-radiative deactivation of the excited state of tertiary amine N-oxide substituted xanthene involving a twisted internal charge transfer (TICT) process and partially due to photo-induced electron transfer (PET) from the N-oxide group. RhoNox-1 showed significant enhancement of the fluorescence signal in Fe2+-loaded cells via selective Fe2+-mediated deoxygenation of the N-oxide group and also successfully detected basal and endogenous labile Fe2+ in living cells.
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