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Vasopressin: A model for the study of effects of additives on the oral and rectal administration of peptide drugs

1988; Elsevier BV; Volume: 77; Issue: 1 Linguagem: Inglês

10.1002/jps.2600770107

1520-6017

Murray Saffran, Craig Bedra, G. Sudesh Kumar, Douglas C. Neckers,

Chemical Reactions and Isotopes

We previously observed, using a relatively primitive assay, that small oral doses (on the order of 1 μg = 1 nmol = 1000 pmol per rat) of vasopressin can produce antidiuresis in hydrated rats, and that the oral activity was enhanced by simultaneous administration of an inhibitor of intestinal proteolysis. A more sensitive semi-automated computer-linked apparatus was used to conveniently and quickly compare the antidiuretic activities of the two natural and one synthetic vasopressin peptides by several routes of administration. (The approximate dose in pmol that resulted in a 50% decrease in urine flow is indicated in square brackets.) Intravenous lysine vasopressin was used as the benchmark dose [5]. Arginine and lysine vasopressin [3500], and the synthetic analogue, 1-deamino-8-D-arginine vasopressin (DDAVP) [20], were active by oral administration. The oral activities of arginine and lysine vasopressin were always enhanced by the simultaneous administration of aprotinin [1000], a natural inhibitor of trypsin; the effect of aprotinin on the oral activity of DDAVP was inconsistent. The vasopressins were more active when administered by the rectum: arginine vasopressin [20] and DDAVP [10]. The rectal activities of the peptides were increased by the absorption adjuvant, 5-methoxysalicylate (arginine vasopressin [10]; DDAVP [0.5]). The vasopressin peptides were also delivered by mouth in an impermeable coating of an azoaromatic cross-linked polymer, which is degraded by bacteria in the colon, to release the peptides in the upper colon for absorption (lysine vasopressin [525]). The use of the vasopressins as model peptide drugs has shown that although the efficacy of peptides is much less by the oral route and somewhat less by the rectal route than by parenteral administration, the effective doses are still very small, indicating that biologically potent peptide drugs can be administered by noninvasive routes.