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A known expressed sequence tag, BM742401, is a potent lincRNA inhibiting cancer metastasis

2013; Springer Nature; Volume: 45; Issue: 7 Linguagem: Inglês

10.1038/emm.2013.59

2092-6413

Seongmin Park, Sung‐Joon Park, Hee‐Jin Kim, Oh‐Hyung Kwon, Tae-Wook Kang, Hyun-Ahm Sohn, Seon‐Kyu Kim, Seung Moo Noh, Kyu‐Sang Song, Se-Jin Jang, Yong Sung Kim, Seon‐Young Kim,

RNA Research and Splicing

Long intergenic non-coding RNAs (lincRNAs) have historically been ignored in cancer biology. However, thousands of lincRNAs have been identified in mammals using recently developed genomic tools, including microarray and high-throughput RNA sequencing (RNA-seq). Several of the lincRNAs identified have been well characterized for their functions in carcinogenesis. Here we performed RNA-seq experiments comparing gastric cancer with normal tissues to find differentially expressed transcripts in intergenic regions. By analyzing our own RNA-seq and public microarray data, we identified 31 transcripts, including a known expressed sequence tag, BM742401. BM742401 was downregulated in cancer, and its downregulation was associated with poor survival in gastric cancer patients. Ectopic overexpression of BM742401 inhibited metastasis-related phenotypes and decreased the concentration of extracellular MMP9. These results suggest that BM742401 is a potential lincRNA marker and therapeutic target. A hunt for abnormally regulated RNA molecules in gastric cancer has revealed a novel prognostic indicator for metastatic invasion. Although they exhibit similar features to messenger RNAs, long intergenic non-coding RNAs (lincRNAs) do not encode proteins but instead act primarily as regulators of other genes. Several lincRNAs have been linked with cancer, and researchers led by Seon-Young Kim of the University of Science and Technology, Korea, have now identified lincRNAs involved with gastric cancer. By combining their own experimental results with previously published gene expression data, the researchers revealed two putative lincRNAs that are abnormally expressed in gastric tumors. One of these, BM742401, modulates tumor metastasis, and the researchers found that low BM742401 levels predict worse outcomes for late-stage gastric cancer. This RNA may therefore offer a useful prognostic biomarker or future clinical target.