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Artigo Acesso aberto Produção Nacional Revisado por pares
BIBTEX RIS

Extraction time for soybean isoflavone determination

2002; Instituto de Tecnologia do Paraná (Tecpar); Volume: 45; Issue: 4 Linguagem: Inglês

10.1590/s1516-89132002000600015

ISSN

1678-4324

Autores

M. C. Carrão-Panizzi, Silvana Pedroso de Goés Favoni, Akio Kikuchi,

Tópico(s)

GABA and Rice Research

Resumo

Studies were carried out on the optimization of the extraction procedures for isoflavones determination in soybean by HPLC. Isoflavones were extracted from 100 mg samples of full fat soybean flour "Kinako" (milled toasted grains). Recovery of average total isoflavones was higher when extraction was performed with agitation(217.2 mg/100g), than without agitation(191.0 mg/100g). Isoflavone extraction without agitation for 1,4, 20 and 24 hours, were equally efficient for total and individual compounds. These results suggested that an efficient isoflavone recovery could be achieved with extraction for one hour with agitation.

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