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Artigo Acesso aberto Produção Nacional Revisado por pares
BIBTEX RIS

Clonal relationships among Escherichia coli serogroup O6 isolates based on RAPD

2006; Oxford University Press; Volume: 148; Issue: 2 Linguagem: Inglês

10.1111/j.1574-6968.1997.tb10297.x

ISSN

1574-6968

Autores

Ana Beatriz Furlanetto Pacheco, Beatriz E.C. Guth, Kellen C.C Soares, Darcy F. de Almeida, Luís Carlos de Souza Ferreira,

Tópico(s)

RNA and protein synthesis mechanisms

Resumo

The genetic diversity in a group of Escherichia coli strains belonging to serogroup O6 but expressing different H antigens was investigated by random amplification of polymorphic DNA (RAPD). Isolates of serotypes H16, H1, H31, and non-motile (NM) strains were typed using a set of 3 primers with different G + C contents. The amplified band arrays allowed the identification of 3 main clonal clusters corresponding to each O:H serotype analyzed. Based on their RAPD profiles NM strains could be assigned to either H1 or H31 serotypes. The results indicate that the flagellar antigen and the RAPD fingerprint represent reliable clonal markers in this E. coli group.

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