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Inhibition of glutathione S-transferases (GSTs) from parasitic nematodes by extracts from traditional Nigerian medicinal plants

2000; Wiley; Volume: 14; Issue: 8 Linguagem: Inglês

10.1002/1099-1573(200012)14

1099-1573

B.B. Fakae, Alison Campbell, J. Barrett, Ian M. Scott, Paul Teesdale‐Spittle, Eva Liebau, Peter M. Brophy,

Genomics, phytochemicals, and oxidative stress

Phytotherapy ResearchVolume 14, Issue 8 p. 630-634 Research Article Inhibition of glutathione S-transferases (GSTs) from parasitic nematodes by extracts from traditional Nigerian medicinal plants B. B. Fakae, B. B. Fakae Department of Veterinary Parasitology and Entomology, University of Nigeria, Nsukka, NigeriaSearch for more papers by this authorA. M. Campbell, A. M. Campbell Institute of Biological Sciences, University of Wales, Aberystwyth SY23 3DA, UKSearch for more papers by this authorJ. Barrett, J. Barrett Institute of Biological Sciences, University of Wales, Aberystwyth SY23 3DA, UKSearch for more papers by this authorI. M. Scott, I. M. Scott Institute of Biological Sciences, University of Wales, Aberystwyth SY23 3DA, UKSearch for more papers by this authorP. H. Teesdale-Spittle, P. H. Teesdale-Spittle Institute of Biological Sciences, University of Wales, Aberystwyth SY23 3DA, UKSearch for more papers by this authorE. Liebau, E. Liebau Department of Biochemical Parasitology, Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Str. 74, D-20359, Hamburg, GermanySearch for more papers by this authorP. M. Brophy, Corresponding Author P. M. Brophy [email protected] Institute of Biological Sciences, University of Wales, Aberystwyth SY23 3DA, UKInstitute of Biological Sciences, University of Wales, Aberystwyth SY23 3DA, UKSearch for more papers by this author B. B. Fakae, B. B. Fakae Department of Veterinary Parasitology and Entomology, University of Nigeria, Nsukka, NigeriaSearch for more papers by this authorA. M. Campbell, A. M. Campbell Institute of Biological Sciences, University of Wales, Aberystwyth SY23 3DA, UKSearch for more papers by this authorJ. Barrett, J. Barrett Institute of Biological Sciences, University of Wales, Aberystwyth SY23 3DA, UKSearch for more papers by this authorI. M. Scott, I. M. Scott Institute of Biological Sciences, University of Wales, Aberystwyth SY23 3DA, UKSearch for more papers by this authorP. H. Teesdale-Spittle, P. H. Teesdale-Spittle Institute of Biological Sciences, University of Wales, Aberystwyth SY23 3DA, UKSearch for more papers by this authorE. Liebau, E. Liebau Department of Biochemical Parasitology, Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Str. 74, D-20359, Hamburg, GermanySearch for more papers by this authorP. M. Brophy, Corresponding Author P. M. Brophy [email protected] Institute of Biological Sciences, University of Wales, Aberystwyth SY23 3DA, UKInstitute of Biological Sciences, University of Wales, Aberystwyth SY23 3DA, UKSearch for more papers by this author First published: 29 November 2000 https://doi.org/10.1002/1099-1573(200012)14:8 3.0.CO;2-5Citations: 45AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Piliostigma thonningii, Ocimum gratissimum, Nauclea latifolia and Alstonia boonei are used in Nigerian traditional medicines against gastrointestinal helminths of animals and man. Proanthocyanidins were detected in Piliostigma and Nauclea, but not Alstonia or Ocimum. Extracts of these plants killed 50% of brine shrimp nauplii at <10 ppm (Nauclea), 100 ppm (Piliostigma) and <1000 ppm (Ocimum and Alstonia), the Nauclea LD50 being similar to the anthelmintic drug piperazine. Extracts were also toxic to the parasitic nematode Haemonchus infective L3 stage. Nematode glutathione-S-transferases (GSTs) are potential drug targets. Apart from Alstonia all the medicinal plants contained heat-stable inhibitory activities against recombinant Ascaris and Onchocerca GSTs in vitro. Piliostigma, Ocimum and Nauclea had IC50s of 2, 10 and 15 µg/mL respectively for Ascaris GST and 4, 8, 28 µg/mL respectively for Onchocerca GST. We suggest that the inhibitory properties of some of these Nigerian plant extracts against GST may contribute to the pharmacological basis of their efficacy against helminths in traditional herbal use. Copyright © 2000 John Wiley & Sons, Ltd. REFERENCES Asuzu IU, Onu OU. 1993. The in vitro acute toxicity of Piliostigma thonningii bark ethanolic extract on selected strongyle larvae of cattle. Fitoterapia 64: 524–528. Asuzu IU, Onu OU. 1994. Anthelmintic activity of the ethanolic extract of Piliostigma thonningii bark in Ascaridia galli infected chickens. Fitoterapia 65: 291–297. Brophy PM, Southan C, Barrett J. 1989. Glutathione transferases in the tapeworm Moniezia expansa. Biochem J 262: 939–946. Brophy PM, Crowley P, Barrett J. 1990. Detoxification reaction of Fasciola hepatica cystolic glutathione transferases. 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