Protective effect of Weikang decoction and partial ingredients on model rat with gastric mucosa ulcer
2005; Baishideng Publishing Group; Volume: 11; Issue: 8 Linguagem: Inglês
10.3748/wjg.v11.i8.1204
ISSN2219-2840
Autores Tópico(s)Pharmacological Effects of Natural Compounds
ResumoAIM:To investigate the protective mechanisms of Weikang (WK) decoction on gastric mucosae. METHODS:Ninety rats were randomly divided into nine groups of 10 each, namely group, model group, group with large WK dosage, group with medium WK dosage, group with small WK dosage, group with herbs of jianpiyiqi (strengthening the spleen and replenishing qi), group with herbs of yangxuehuoxue (invigorating the circulation of and nourishing the blood), group with herbs of qingrejiedu (clearing away the heat-evils and toxic materials), group with colloidal bismuth pectin (CBP) capsules.According to the method adopted by Yang Xuesong, except normal control group, chronic gastric ulcer was induced with 100% acetic acid.On the sixth day after moldmaking, WK decoction was administered, respectively at doses of 20, 10 and 5 g/kg to rats of the WK groups, or the groups with herbs of jianpiyiqi, yangxuehuoxue and qingrejiedu, 10 mL/kg was separately administered to each group every day.For the group with CBP capsules, medicine was dissolved with water and doses 15 times of human therapeutic dose were administered (10 mL/kg solution containing 0.35% CBP).Rats of other groups were fed with physiological saline (10 mL/kg every day).Administration lasted for 16 d.Rats were killed on d 22 after mold making to observe changes of gastric mucosa.The mucus thickness of gastric mucosa surface was measured.Levels of epidermal growth factor (EGF) in gastric juice, nitric oxide (NO) in gastric tissue, endothelin (ET) in plasma, superoxide dismutase (SOD) in plasma, malondialdehyde (MDA) in plasma and prostaglandin I 2 (PGI 2 ) were examined. RESULTS:Compared with control group, ulceration was found in gastric mucosa of model group rats.The mucus thickness of gastric mucosa surface, the levels of EGF, NO, 6-K-PGF 1 and SOD decreased significantly in the model group (EGF: 0.818±0.18vs 2.168±0.375,NO: 0.213±0.049vs 0.601±0.081,6-K-PGF 1 : 59.7±6.3 vs 96.6±8.30,SOD: 128.6±15.0vs 196.6±35.3,P<0.01), the levels of ET (179.96±37.40vs 46.64±21.20,P<0.01) and MDA (48.2±4.5 vs 15.7±4.8,P<0.01) increased.Compared with model group, the thickness of regenerative mucosa increased, glandular arrangement was in order, and cystic dilative glands decreased, while the mucus thickness of gastric mucosa surface increased (20 g/kg WK: 51.3±2.9
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