Immunoglobulin heavy‐chain gene rearrangement in peripheral blood mononuclear cells in non‐Hodgkin's lymphomas ‐ Correlation with kappa:lambda analysis and clinical features
1989; Wiley; Volume: 42; Issue: 2 Linguagem: Inglês
10.1111/j.1600-0609.1989.tb01202.x
ISSN1600-0609
AutoresJack Lindh, Anita Lindström, Per Lenner, Erik Lundgren, Göran Roos,
Tópico(s)Chronic Myeloid Leukemia Treatments
Resumo41 patients with non‐Hodgkin's lymphomas were analysed to determine occurrence of B‐cell monoclonality in peripheral blood mononuclear cells using two different methods: determination of kappa:lambda ratio by light microscopic immunofluorescence, and heavy‐chain gene rearrangement by DNA‐technique. In 21 patients (51%) clonal heavy‐chain rearrangement was found in blood, whilst 18 of the patients (44%) showed and abnormal kappa:lambda ratio. Discordant results between the methods were observed in 5 cases. Clones with gene rearrangements suggesting blood involvement were found in 16/25 (64%) patients with low grade lymphomas, in 5/16 (31%) patients with high grade lymphoma, in 17/21 (81%) patients with bone marrow involvement, in 20/27 (74%) of stage III‐IV lymphomas and in all of the 14 patients with high lymphocyte count (≥ 5.0 times 10 9 ). The conclusion was that clonal analysis by the DNA‐technique is a more sensitive method than the kappa:lambda determination using immunofluorescence. Even though the method is time‐consuming, it could prove to be valuable in selected cases.
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