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A Surface Plasmon Resonance Biochip That Operates Both in the Angular and Wavelength Interrogation Modes

2013; Institute of Electrical and Electronics Engineers; Volume: 62; Issue: 5 Linguagem: Inglês

10.1109/tim.2012.2232433

1557-9662

Leiva Casemiro Oliveira, Cleumar da Silva Moreira, Carsten Thirstrup, Elmar U. K. Melcher, A.M.N. Lima, Helmut Neff,

Plasmonic and Surface Plasmon Research

This paper presents a surface plasmon resonance system based on a polymer prism chip. The device allows operation in both the angular and wavelength interrogation modes. The biochip design is discussed emphasizing the effect of the ambient temperature over the optical behavior. Birefringence effect, biochip polishing, and responsivity are also reported. The basic mathematical formulation for both operating modes is discussed, and morphological parameters are considered in the data analysis. Experimental sensorgrams obtained at both interrogation modes with the same polymer prism chip are presented and compared. The experimental sensorgrams obtained with assays providing reversible (phosphate buffered saline and hypochlorite solutions) and irreversible (neutravindin solution) bindings demonstrate the feasibility of the proposed design.