A monoclonal antibody based solid-phase enzyme-binding assay to measure levels of placental alkaline phosphatase in serum of women during pregnancy
1985; Elsevier BV; Volume: 79; Issue: 1 Linguagem: Inglês
10.1016/0022-1759(85)90396-5
ISSN1872-7905
AutoresS.F. Contractor, Eileen Holmes-Ievers, Adrienne Morgan, M. Oakey, N A Staines,
Tópico(s)Celiac Disease Research and Management
ResumoMonoclonal antibodies raised against highly purified placental alkaline phosphatase (PALP) reacted specifically with PALP and were found not to cross-react with the liver, kidney and intestinal enzymes. One of these antibodies (AP-3) was selected to develop an enzyme-binding assay to measure levels of PALP in the sera of 11 women throughout pregnancy. PALP activity rose sharply after 28 weeks of gestation reaching a peak at delivery followed by an immediate drop within 4 days to values approaching non-pregnant levels. The potential of using this assay as an index of placental function to monitor progress of a pregnancy is discussed.
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