Multiple Sample PCR Amplification and Electrophoretic Analysis on a Microchip
1998; American Chemical Society; Volume: 70; Issue: 24 Linguagem: Inglês
10.1021/ac980447e
ISSN1520-6882
AutoresLarry C. Waters, Stephen C. Jacobson, Natalia Kroutchinina, Julia Khandurina, Robert S. Foote, J. Michael Ramsey,
Tópico(s)Microfluidic and Bio-sensing Technologies
ResumoPolymerase chain reactions (PCRs) were carried out on as many as four DNA samples at a time on a microchip device. The PCR products were then analyzed, either individually or together on the same device, by microchip gel electrophoresis. A standard PCR protocol was used to amplify 199- and 500-base pair (bp) regions of bacteriophage λ DNA and 346- and 410- bp regions of E. coli genomic and plasmid DNAs, respectively. Thermal lysis of the bacteria was integrated into the PCR cycle. A product sizing medium, poly(dimethylacrylamide), and an intercalating dye for fluorescence detection were used in the electrophoretic analysis of the products. PCR product sizes were determined by coelectrophoresis with marker DNA.
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