The Dynamic Impact of CpG Methylation in DNA
2000; American Chemical Society; Volume: 39; Issue: 16 Linguagem: Inglês
10.1021/bi9917636
ISSN1943-295X
AutoresKaren B. Geahigan, Gary A. Meints, Mary E. Hatcher, John Orban, Gary P. Drobny,
Tópico(s)Advanced NMR Techniques and Applications
ResumoSolid-state deuterium NMR is used to investigate perturbations of the local, internal dynamics in the EcoRI restriction binding site, -GAATTC- induced by cytidine methylation. Methylation of the cytidine base in this sequence is known to suppress hydrolysis by the EcoRI restriction enzyme. Previous solid-state deuterium NMR studies have detected large amplitude motions of the phosphate-sugar backbone at the AT-CG junction of the unmethylated DNA sequence. This study shows that methylation of the cytidine base in a CpG dinucleotide reduces the amplitudes of motions of the phosphate-sugar backbone. These observations suggest a direct link between suppression of the amplitudes of localized, internal motions of the sugar-phosphate backbone of the DNA and inhibition of restriction enzyme cleavage.
Referência(s)