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EFFECT OF ION REMOVAL ON THE SOLUBILITY OF RAT BRAIN PROTEINS IN CHLOROFORM‐METHANOL MIXTURES*

1968; Wiley; Volume: 15; Issue: 3 Linguagem: Inglês

10.1111/j.1471-4159.1968.tb06190.x

1471-4159

Marjorie B. Lees,

Metabolomics and Mass Spectrometry Studies

Abstract —The amount of chloroform‐methanol‐soluble protein obtained from rat brain tissue homogenates which have been subjected to washing by repeated contrifugation or dialysis is a several‐fold greater than that obtained from untreated homogenates. The increase consequence of the removal of loosely bound electrolytes during the process of centrifugation or dialysis: little or no increase is observed upon (a) the addition of the supernatant, the ashed supernatant, or the diffusate to the washed homogenate; (b) the addition of inorganic salts to the washed homogenate; or (c) washing of the homogenate with a salt solution. The previously observed effect of sucrose in increasing the amount of chloroform–methanol‐soluble protein obtained from subcellular fractions is apparently superimposed upon the effect of the removal of salts.