The Preparation Of Xylulose 5-Phosphate Using Transketolase

1973; Marcel Dekker; Volume: 3; Issue: 6 Linguagem: Inglês

10.1080/00327487308061533

ISSN

2331-0510

Autores

Terry Wood,

Tópico(s)

Enzyme Structure and Function

Resumo

Abstract The condensation of D-glyceraldehyde 3-phosphate and hydroxypyruvate by transketolase to form D-xylulose 5-phosphate was investigated. Apparent Km values of 70 μM for D-glyceraldehyde phosphate and 33 mM for hydroxypyruvate were determined. Procedures for carrying out the reaction and isolating the product are described.

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