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Elution of human satellite DNAs on a methylated albumin kieselguhr chromatographic column:Isolation of satellite DNA IV

1972; Elsevier BV; Volume: 269; Issue: 2 Linguagem: Inglês

10.1016/0005-2787(72)90427-3

1879-3002

Gianmarco Corneo, Luciano Zardi, E. Polli,

Advanced biosensing and bioanalysis techniques

Human DNA has been eluted on a methylated albumin kieselguhr column: the fractions obtained have been centrifuged in Ag+-Cs2SO4 to show the presence of satellite DNAs. Satellite DNA II (density in CsCl = 1.693 g/ml) and the new satellite DNA IV (density in CsCl = 1.700 g/ml) are eluted early from the column and band, respectively, on the heavy side and the light side compared to the main DNA band in Ag+-Cs2SO4. Satellite DNA I (density in CsCl = 1.687 g/ml) and III (density in CsCl = 1.696 g/ml) are eluted late from the column. Satellite DNA IV separates into the complementary strands displaying a difference in density of 0.012 g/ml in alkaline CsCl and renatures rapidly after heat denaturation.