Automation of the Batch Method for Reaction Kinetic Studies Using Flow Injection Analysis. Kinetic Study of Hydrolysis Of N 4 -Acetylsulfanilamide, Acetylsalicylic Acid and Phenyl Phosphate
1992; Taylor & Francis; Volume: 25; Issue: 12 Linguagem: Inglês
10.1080/00032719208017964
ISSN1532-236X
AutoresMichael Α. Koupparis, Paraskevi I. Anagnostopoulou, Constantinos A. Georgiou, T.P. Hadjiioannou,
Tópico(s)Chemical Reaction Mechanisms
ResumoAbstract The automation of the discontinuous (batch) method for kinetic stucfies using flow-injection analysis (FIA) is described. Aliquots of the reaction mixture are automatically injected in an appropriate manifold and the kinetic profile of the reaction is obtained as a series of absorbance peaks. Observed reaction rate constants are calculated using the Guggenheim and non-linear fitting approaches. The new method is evaluated in the kinetic study of the acid hydrolysis of N4-acetylsulfanilamide by colorimetric monitoring of sulfanilamide, the alkaline hydrolysis of acetylsalicylic acid (aspirin) by colorimetric monitoring of salicylate, and the enzymic hydrolysis of phenyl phosphate with alkaline phosphatase by colorimetric monitoring of phosphate. The automated flow-injection batch method can be used in kinetic studies of reactions with t1/2 greater than 200 s.
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