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Extemporaneous preparation of large unilamellar liposomes

1985; Elsevier BV; Volume: 821; Issue: 1 Linguagem: Inglês

10.1016/0005-2736(85)90156-7

1879-2642

Jean R. Philippot, S. Mutaftschiev, Jean‐Pierre Liautard,

Mass Spectrometry Techniques and Applications

Direct contact between lipids solubilized by octyl glucoside and Amberlite XAD-2 beads yielded large liposomes (240 nm diameter) with no residual detergent molecules, in less than 10 min. This extemporaneous preparation of liposomes was prepared with a detergent/bead ratio no higher than 0.12 (μmol/mg) and a phosphatidylcholine/phosphatidylserine/cholesterol molar ratio of 1:1:1. The liposomes were mainly unilamellar, as deduced from thin section and freeze-fracture electron micrographs and from measurement of calcein incorporation into the vesicles. The relatively large internal volume of these vesicles (8.9 l/mol lipid) accounts for the high percentage of entrapped material observed. The percentage increased with lipid concentration, but could not be increased above 20% corresponding to 20 mM total lipids.