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Hydroxyproline quantification for the estimation of collagen in tissue using multiple reaction monitoring mass spectrometry

2008; Elsevier BV; Volume: 1212; Issue: 1-2 Linguagem: Inglês

10.1016/j.chroma.2008.10.011

1873-3778

Michelle L. Colgrave, Peter Allingham, Alun Jones,

Protein Hydrolysis and Bioactive Peptides

Collagens are highly abundant mammalian proteins that contain a high content of hydroxylated amino acids such as hydroxyproline. We have exploited the high hydroxyproline content of collagen and developed a method for hydroxyproline quantification as a measure of collagen content in muscle samples. The novel method utilizes a highly selective and sensitive method of multiple reaction monitoring (MRM) by mass spectrometry. The analytical method is simple, rapid (5 min), convenient (no derivatization), precise ( 0.999) over three orders of magnitude (5–5000 nmol/L).