DNA Damage-induced Translocation of the Werner Helicase Is Regulated by Acetylation
2002; Elsevier BV; Volume: 277; Issue: 52 Linguagem: Inglês
10.1074/jbc.m210479200
ISSN1083-351X
AutoresGil Blander, Noa Zalle, Yaron Daniely, Jan Taplick, Matthew D. Gray, Moshe Oren,
Tópico(s)Genomics and Chromatin Dynamics
ResumoWerner syndrome is a rare autosomal recessive disorder involving the premature appearance of features reminiscent of human aging. Werner syndrome occurs by mutation of the WRN gene, encoding a DNA helicase. WRN contributes to the induction of the p53 tumor suppressor protein by various DNA damaging agents. Here we show that UV exposure leads to extensive translocation of WRN from the nucleolus to nucleoplasmic foci in a dose-dependent manner. Ionizing radiation also induces WRN translocation, albeit milder, partially through activation of the ATM kinase. The nucleoplasmic foci to which WRN is recruited display partial colocalization with PML nuclear bodies. The translocation of WRN into nucleoplasmic foci is significantly enhanced by the protein deacetylase inhibitor, Trichostatin A. Moreover, Trichostatin A delays the re-entry of WRN into the nucleolus at late times after irradiation. WRN is acetylatedin vivo, and this is markedly stimulated by the acetyltransferase p300. Importantly, p300 augments the translocation of WRN into nucleoplasmic foci. These findings support the notion that WRN plays a role in the cellular response to DNA damage and suggest that the activity of WRN is modulated by DNA damage-induced post-translational modifications of WRN and possibly WRN-interacting proteins. Werner syndrome is a rare autosomal recessive disorder involving the premature appearance of features reminiscent of human aging. Werner syndrome occurs by mutation of the WRN gene, encoding a DNA helicase. WRN contributes to the induction of the p53 tumor suppressor protein by various DNA damaging agents. Here we show that UV exposure leads to extensive translocation of WRN from the nucleolus to nucleoplasmic foci in a dose-dependent manner. Ionizing radiation also induces WRN translocation, albeit milder, partially through activation of the ATM kinase. The nucleoplasmic foci to which WRN is recruited display partial colocalization with PML nuclear bodies. The translocation of WRN into nucleoplasmic foci is significantly enhanced by the protein deacetylase inhibitor, Trichostatin A. Moreover, Trichostatin A delays the re-entry of WRN into the nucleolus at late times after irradiation. WRN is acetylatedin vivo, and this is markedly stimulated by the acetyltransferase p300. Importantly, p300 augments the translocation of WRN into nucleoplasmic foci. These findings support the notion that WRN plays a role in the cellular response to DNA damage and suggest that the activity of WRN is modulated by DNA damage-induced post-translational modifications of WRN and possibly WRN-interacting proteins. Werner syndrome Trichostatin A green fluorescence protein proliferating cell nuclear antigen ataxia telangiectasia mutation ionizing radiation 4′,6-diamidino-2-phenylindole hydrochloride Werner syndrome, a rare autosomal recessive disorder, is considered a useful model for investigating human aging (1Shen J. Loeb L.A. Mech. Ageing Dev. 2001; 122: 921-944Crossref PubMed Scopus (82) Google Scholar). Werner syndrome (WS)1 patients exhibit a wide range of features typical of human aging and in addition are highly predisposed to the emergence of benign and malignant neoplasms (2Goto M. Miller R.W. Ishikawa Y. Sugano H. Cancer Epidemiol. Biomarkers Prev. 1996; 5: 239-246PubMed Google Scholar). Cultured cells from WS patients exhibit a shortened life span and a prolonged S-phase of the cell cycle (3Poot M. Hoehn H. Runger T.M. Martin G.M. Exp. Cell Res. 1992; 202: 267-273Crossref PubMed Scopus (186) Google Scholar). WS occurs by mutation of the WRN gene, a member of the RecQ DNA helicase family (4Yu C.E. Oshima J., Fu, Y.H. Wijsman E.M. Hisama F. Alisch R. Matthews S. Nakura J. Miki T. Ouais S. Martin G.M. Mulligan J. Schellenberg G.D. Science. 1996; 272: 258-262Crossref PubMed Scopus (1473) Google Scholar). There are five known human recQ helicases (for recent review, see Ref.5Mohaghegh P. Hickson I.D. Hum. Mol. Genet. 2001; 10: 741-746Crossref PubMed Scopus (186) Google Scholar). Mutations in two other family members, BLM and RecQ4, are responsible for the premature aging syndromes, Bloom's (6Ellis N.A. Groden J., Ye, T.Z. Straughen J. Lennon D.J. Ciocci S. Proytcheva M. German J. Cell. 1995; 83: 655-666Abstract Full Text PDF PubMed Scopus (1200) Google Scholar) and Rothmund Thomson (7Kitao S. Shimamoto A. Goto M. Miller R.W. Smithson W.A. Lindor N.M. Furuichi Y. Nat. Genet. 1999; 22: 82-84Crossref PubMed Scopus (565) Google Scholar), respectively. WRN is a double-stranded DNA unwinding enzyme that additionally possesses exonuclease activity (8Gray M.D. Shen J.C. Kamath-Loeb A.S. Blank A. Sopher B.L. Martin G.M. Oshima J. Loeb L.A. Nat. Genet. 1997; 17: 100-103Crossref PubMed Scopus (515) Google Scholar, 9Huang S., Li, B. Gray M.D. Oshima J. Mian I.S. Campisi J. Nat. Genet. 1998; 20: 114-116Crossref PubMed Scopus (372) Google Scholar, 10Shen J.C. Loeb L.A. Nucleic Acids Res. 2000; 28: 3260-3268Crossref PubMed Scopus (80) Google Scholar, 11Bohr V.A. Cooper M. Orren D. Machwe A. Piotrowski J. Sommers J. Karmakar P. Brosh R. Exp. Gerontol. 2000; 35 (6–7): 695-702Crossref PubMed Scopus (36) Google Scholar, 12Oshima J. Bioessays. 2000; 22: 894-901Crossref PubMed Scopus (69) Google Scholar). In normal human fibroblasts, WRN steady state protein levels are relatively constant throughout the cell cycle, and the WRN protein is localized mainly to the nucleolus (13Gray M.D. Wang L. Youssoufian H. Martin G.M. Oshima J. Exp. Cell Res. 1998; 242: 487-494Crossref PubMed Scopus (124) Google Scholar, 14Marciniak R.A. Lombard D.B. Johnson F.B. Guarente L. Proc. Natl. Acad. Sci. U. S. A. 1998; 95: 6887-6892Crossref PubMed Scopus (179) Google Scholar). Following treatment of cells with 4NQO, an alkylating agent, WRN is no longer detected in the nucleolus (13Gray M.D. Wang L. Youssoufian H. Martin G.M. Oshima J. Exp. Cell Res. 1998; 242: 487-494Crossref PubMed Scopus (124) Google Scholar). WRN was found to interact directly with numerous proteins, including proliferating cell nuclear antigen and Topoisomerase I (15Lebel M. Spillare E.A. Harris C.C. Leder P. J. Biol. Chem. 1999; 274: 37795-37799Abstract Full Text Full Text PDF PubMed Scopus (207) Google Scholar), Replication Protein A (RPA) (16Brosh R.M., Jr. Orren D.K. Nehlin J.O. Ravn P.H. Kenny M.K. Machwe A. Bohr V.A. J. Biol. Chem. 1999; 274: 18341-18350Abstract Full Text Full Text PDF PubMed Scopus (255) Google Scholar), p53 (17Spillare E.A. Robles A.I. Wang X.W. Shen J.C., Yu, C.E. Schellenberg G.D. Harris C.C. Genes Dev. 1999; 13: 1355-1360Crossref PubMed Scopus (153) Google Scholar, 18Blander G. Kipnis J. Leal J.F., Yu, C.E. Schellenberg G.D. Oren M. J. Biol. Chem. 1999; 274: 29463-29469Abstract Full Text Full Text PDF PubMed Scopus (152) Google Scholar), the Ku complex (19Cooper M.P. Machwe A. Orren D.K. Brosh R.M. Ramsden D. Bohr V.A. Genes Dev. 2000; 14: 907-912PubMed Google Scholar), and DNA Pol δ (20Szekely A.M. Chen Y.H. Zhang C. Oshima J. Weissman S.M. Proc. Natl. Acad. Sci. U. S. A. 2000; 97: 11365-11370Crossref PubMed Scopus (102) Google Scholar). In this regard, WRN has been suggested to be a functional component of various cellular processes, including DNA replication, transcription, recombination, and repair. However, the precise physiological role of WRN remains to be elucidated. DNA damage alters the subcellular localization of many proteins, as exemplified by ING1 (21Scott M. Boisvert F.M. Vieyra D. Johnston R.N. Bazett-Jones D.P. Riabowol K. Nucleic Acids Res. 2001; 29: 2052-2058Crossref PubMed Scopus (99) Google Scholar), 53BP1 (22Rappold I. Iwabuchi K. Date T. Chen J. J. Cell Biol. 2001; 153: 613-620Crossref PubMed Scopus (396) Google Scholar), and BLM (23Bischof O. Kim S.H. Irving J. Beresten S. Ellis N.A. Campisi J. J. Cell Biol. 2001; 153: 367-380Crossref PubMed Scopus (233) Google Scholar). The effects of DNA damage are often exerted through post-translational modification of target proteins, as shown for instance for p53 and Mdm2 (24Meek D.W. Oncogene. 1999; 18: 7666-7675Crossref PubMed Scopus (208) Google Scholar, 25Appella E. Anderson C.W. Eur. J. Biochem. 2001; 268: 2764-2772Crossref PubMed Scopus (899) Google Scholar, 26Liang S.H. Clarke M.F. Eur. J. Biochem. 2001; 268: 2779-2783Crossref PubMed Scopus (160) Google Scholar, 27Woods D.B. Vousden K.H. Exp. Cell Res. 2001; 264: 56-66Crossref PubMed Scopus (288) Google Scholar, 28Maya R. Balass M. Kim S.T. Shkedy D. Leal J.F. Shifman O. Moas M. Buschmann T. Ronai Z. Shiloh Y. Kastan M.B. Katzir E. Oren M. Genes Dev. 2001; 15: 1067-1077Crossref PubMed Scopus (523) Google Scholar). Although most of the work addressing the role of post-translational modifications in the DNA damage response has focused on protein phosphorylation, there is now growing evidence that protein acetylation may also play an important role (25Appella E. Anderson C.W. Eur. J. Biochem. 2001; 268: 2764-2772Crossref PubMed Scopus (899) Google Scholar, 29Brand M. Moggs J.G. Oulad-Abdelghani M. Lejeune F. Dilworth F.J. Stevenin J. Almouzni G. Tora L. EMBO J. 2001; 20: 3187-3196Crossref PubMed Scopus (176) Google Scholar, 30Grossman S.R. Eur. J. Biochem. 2001; 268: 2773-2778Crossref PubMed Scopus (188) Google Scholar, 31Hasan S. Stucki M. Hassa P.O. Imhof R. Gehrig P. Hunziker P. Hubscher U. Hottiger M.O. Mol. Cell. 2001; 7: 1221-1231Abstract Full Text Full Text PDF PubMed Scopus (140) Google Scholar). Furthermore, acetylation can modulate the function of many proteins, their interaction with other proteins, and their subcellular localization (25Appella E. Anderson C.W. Eur. J. Biochem. 2001; 268: 2764-2772Crossref PubMed Scopus (899) Google Scholar, 27Woods D.B. Vousden K.H. Exp. Cell Res. 2001; 264: 56-66Crossref PubMed Scopus (288) Google Scholar,32Chan H.M. Krstic-Demonacos M. Smith L. Demonacos C. La Thangue N.B. Nat. Cell Biol. 2001; 3: 667-674Crossref PubMed Scopus (237) Google Scholar, 33Roth S.Y. Denu J.M. Allis C.D. Annu. Rev. Biochem. 2001; 70: 81-120Crossref PubMed Scopus (1562) Google Scholar, 34Soutoglou E. Katrakili N. Talianidis I. Mol. Cell. 2000; 5: 745-751Abstract Full Text Full Text PDF PubMed Scopus (239) Google Scholar, 35Spilianakis C. Papamatheakis J. Kretsovali A. Mol. Cell. Biol. 2000; 20: 8489-8498Crossref PubMed Scopus (134) Google Scholar). To explore possible links between WRN and the DNA damage response, we investigated the effect of several types of DNA damage on the subcellular distribution of WRN. We report that in response to UV exposure, WRN translocates from the nucleolus to nucleoplasmic foci. The rate and extent of WRN redistribution are correlated with the UV dose. Importantly, p300 promotes WRN acetylation in vivo, while cotransfection with p300 or treatment with Trichostatin A (TSA), a deacetylase inhibitor, mimics the effect of DNA damage and causes translocation of WRN to discrete nucleoplasmic foci. Similarly, TSA inhibits the reentry of WRN from such foci back into the nucleolus at late times after irradiation. The nucleoplasmic foci to which WRN is recruited upon irradiation or TSA treatment exhibit partial colocalization with PML nuclear bodies, which are known to contain protein acetyltransferases. Together, these findings support the notion that WRN may play a role in the DNA damage response pathway, perhaps in signaling or in facilitating specific types of DNA repair, in a manner that is regulated by protein acetylation. WI-38 normal human fibroblasts, as well as fibroblasts from individuals diagnosed as WS patients (AG00780), were obtained from the National Institute of General Medical Sciences Human Genetic Mutant Cell Repository (Coriell Institute, Camden, NJ). U2OS human osteosarcoma cells were maintained in Dulbecco's modified Eagle's medium containing 10% fetal calf serum. Human fibroblasts were maintained in Eagle's minimum essential medium containing 20% fetal calf serum. Cells were incubated at 37.0 °C in 5% CO2. The GFP-WRN expression plasmid was a kind gift from Dr. S. M. Weissman (36Hisama F.M. Chen Y.H. Meyn M.S. Oshima J. Weissman S.M. Cancer Res. 2000; 60: 2372-2376PubMed Google Scholar). Transient transfection was performed by the calcium phosphate method as previously described (18Blander G. Kipnis J. Leal J.F., Yu, C.E. Schellenberg G.D. Oren M. J. Biol. Chem. 1999; 274: 29463-29469Abstract Full Text Full Text PDF PubMed Scopus (152) Google Scholar). For UV irradiation, U2OS cells (300,000 per 60-mm dish) or human fibroblasts (60,000 per 35-mm dish) were washed with phosphate-buffered saline and irradiated in a UV Stratalinker 1800 (Stratagene), predominantly at 254 nm. Phosphate-buffered saline was then replaced with fresh medium. For ionizing radiation (IR) treatment, cells were exposed to a GB-150 cobalt-60 (Nordion Canada) γ-irradiation source. Cells were irradiated in their original medium. Camptothecin (CPT) and 4NQO (both from Sigma) were dissolved in dimethyl sulfoxide (Me2SO) and used at 1 μg/ml and 0.2 μg/ml, respectively. TSA (Upstate) was dissolved in Me2SO and used at 100 ng/ml. The anti-WRN polyclonal antibody was used for immunofluorescence and confocal analysis as previously described (13Gray M.D. Wang L. Youssoufian H. Martin G.M. Oshima J. Exp. Cell Res. 1998; 242: 487-494Crossref PubMed Scopus (124) Google Scholar). Images were acquired as single scans on a Zeiss confocal microscope using LSM software. Antibody against PML was a kind gift from Dr. A. Dejean. To detect WRN acetylationin vivo, 293T cells (2 × 106 per 10-cm dish) were transfected by the JetPEI method. WRN plasmid (3 μg) was transfected either alone or in combination with a p300 expression plasmid (7 μg). 48 h after transfection, 2 mci/ml [3H]sodium acetate, TSA(10 μm), and the proteasomal inhibitor MG132 (Sigma, 25 μm) were added to the culture for 1 h. Cells were then washed twice with cold phosphate-buffered saline and lysed in Nonidet P-40 lysis buffer with freshly added protease inhibitor mixture (Sigma). The lysate was centrifuged at 17,000 × g for 15 min at 4 °C. Supernatants were incubated with anti-WRN polyclonal serum, previously conjugated to protein A-Sepharose beads, for 1 h at 4 °C. The beads were washed three times with 1 ml of lysis buffer. Immunoprecipitated proteins were resolved on a 7.5% SDS-PAGE gel. Gels containing [3H]acetate-labeled WRN were first stained with Coomassie Brilliant Blue and then enhanced by impregnating with a commercial fluorography enhancing solution (Amplify, AmershamBiosciences) for 30 min. Dried gels were subjected to autoradiography at −70 °C for 3–7 days. To investigate the effects of DNA damage on the subcellular localization of WRN, human osteosarcoma U2OS cells were transfected transiently with a plasmid encoding a fusion protein between WRN and green fluorescent protein (GFP). In agreement with what has been reported previously for endogenous WRN (13Gray M.D. Wang L. Youssoufian H. Martin G.M. Oshima J. Exp. Cell Res. 1998; 242: 487-494Crossref PubMed Scopus (124) Google Scholar), GFP-WRN was localized almost exclusively to the nucleolus (Fig. 1 A), as confirmed by staining for the nucleolar protein nucleophosmin/B23 (Fig. 1 C). Exposure of the cells to high dose UV radiation (40J/m2) resulted in translocation of GFP-WRN from the nucleolus into a large number of small nucleoplasmic foci (Fig. 1 D). A similar pattern of GFP-WRN translocation was also seen after treatment with either camptothecin (1 μg/ml) or 4NQO (0.2 μg/ml) (data not shown). Exposure to IR (10 Gray), had a milder effect with nucleoplasmic translocation seen in only some of the cells (Fig. 1 F). Unlike the simple kinetics of induction by UV (Fig. 1 F) or camptothecin (data not shown), IR elicited a biphasic response: a very rapid wave of GFP-WRN exit from the nucleolus followed by a slower, extended, and rather limited second wave (Fig. 1 F). Endogenous WRN protein manifested an essentially similar behavior. Thus, in untreated WI-38 diploid human fibroblasts (Fig. 2 A), the vast majority of endogenous WRN was in the nucleolus, in agreement with earlier observations (13Gray M.D. Wang L. Youssoufian H. Martin G.M. Oshima J. Exp. Cell Res. 1998; 242: 487-494Crossref PubMed Scopus (124) Google Scholar). A variable amount of nucleoplasmic staining could also be observed under those conditions, typically residing in diffuse small foci (Fig. 2 A). This indicates that a minor fraction of WRN is present outside the nucleolus even without induced stress. Upon UV irradiation, WRN was effectively excluded from the nucleolus and translocated entirely to nucleoplasmic foci (Fig. 2 C). Similar results were obtained with camptothecin and 4NQO (data not shown). A more detailed time course analysis is shown in Fig. 3. In untreated cells, GFP-WRN was exclusively in the nucleolus (Fig. 3 A). Shortly after irradiation at 40J/m2, some of the GFP-WRN was still retained in the nucleolus, whereas the rest of the protein had already translocated into the nucleoplasm and was visible either in a relatively small number of large foci or as diffuse nucleoplasmic staining (Fig. 3, E and F). Later on, WRN became undetectable in the nucleoli, and much of the protein was found in a large number of small foci distributed throughout the nucleoplasm (Fig. 3 G; quantitative analysis shown in Fig. 3 H). At later times after irradiation, the GFP-WRN protein began to reaccumulate in the nucleoli, such that 24 h postirradiation the majority of the cells again exhibited only nucleolar GFP-WRN (see Fig. 5 A, panel e).Figure 5Trichostatin A regulates the subnuclear localization of GFP-WRN. A, U2OS cells were transiently transfected with a GFP-WRN expression plasmid. 24 h post-transfection, TSA (c, d, g,h) or Me2SO as a solvent control (a,b, e, f) was added for an additional 30 h, at which time the cells were fixed and stained with DAPI (b, d, f, h). GFP-WRN was monitored as in Fig. 1 (a, c, e,g). In e–h, cells were exposed to 20J/m2 UV 6 h after the addition of TSA or Me2SO, such that the experiment was terminated 24 h after irradiation. B, quantitative analysis of the percentage of cells exhibiting prominent nucleoplasmic localization of GFP-WRN as a function of culture history (see panel A). C, WI-38 cells were incubated for 30 h with TSA or with Me2SO as a control (Ctrl) before being processed as in Fig. 2. Shown is a quantitative analysis of the percentage of cells in which the endogenous WRN protein was exclusively in the nucleoplasm.View Large Image Figure ViewerDownload Hi-res image Download (PPT) The extent and pattern of WRN relocalization depended on the dose of radiation. Thus, in cells exposed to 3J/m2 UV, much of the GFP-WRN was retained in the nucleolus, whereas the remainder was present in a relatively small number of rather large nucleoplasmic foci (Fig. 3 B), similar to the picture seen very early after exposure to a high dose of UV irradiation. This limited translocation reached a maximum rapidly and did not progress significantly beyond 30 min postirradiation (Fig. 3, B–D; quantitative analysis in Fig. 3 H). The correlation between UV dose and amount of nucleoplasmic WRN foci supports a role for WRN in the DNA damage response that ensues upon exposure of the cells to genotoxic agents. The progressive translocation following high dose irradiation may reflect the inability of the cell to deal successfully with such extensive DNA damage as fast as it does with a limited amount of damage. The rapid DNA damage response elicited by IR is mediated, to a large extent, by the ATM kinase (37Shiloh, Y. (2002) Ernst Schering Res. Found. Workshop 36, 51–70Google Scholar). To determine whether this was also the case for WRN translocation, the effect of IR on WRN localization was compared between normal human fibroblasts and cells derived from ataxia telangiectasia patients lacking ATM function. Detailed kinetic analysis revealed that in ATM-deficient cells, the first rapid phase of WRN translocation was completely abrogated (Fig. 4). This finding suggests that ATM can contribute to the initial response to IR-induced DNA damage by catalyzing the rapid mobilization of WRN into the nucleoplasm. It is conceivable that the extended, slow second phase of WRN translocation, which remains unaffected in ATM-deficient cells, may be due to the action of the ATM-related ATR kinase, although this remains to be formally proven. The intracellular localization of proteins can be modulated by a variety of post-translational modifications. This also relates to protein acetylation, as exemplified by hepatocyte nuclear factor 4 (34Soutoglou E. Katrakili N. Talianidis I. Mol. Cell. 2000; 5: 745-751Abstract Full Text Full Text PDF PubMed Scopus (239) Google Scholar). Furthermore, changes in acetylation in response to DNA damage can also modulate the functional state of proteins, as exemplified by p53 (25Appella E. Anderson C.W. Eur. J. Biochem. 2001; 268: 2764-2772Crossref PubMed Scopus (899) Google Scholar, 30Grossman S.R. Eur. J. Biochem. 2001; 268: 2773-2778Crossref PubMed Scopus (188) Google Scholar). We therefore investigated whether protein acetylation may play a role in the observed relocalization of WRN. To that end, U2OS cells were transfected with GFP-WRN and incubated for 30 h in the presence of the histone deacetylase inhibitor TSA. As shown in Fig. 5 A, panel c, TSA induced a prominent translocation of GFP-WRN from the nucleolus into discrete, rather large nucleoplasmic foci, similar to those observed early after exposure to high dose UV. Moreover, TSA also blocked the return of GFP-WRN from the nucleoplasmic foci back into the nucleolus. Thus, although 24 h after exposure to high dose UV the majority of the cells again displayed only nucleolar WRN, treatment with TSA significantly slowed this return and maintained much of the WRN in nucleoplasmic foci for longer periods (Fig. 5 A, panels e and g, and Fig. 5 B). These findings indicate that protein acetylation, augmented by TSA, plays a positive role in enabling the translocation of WRN from the nucleolus to nucleoplasmic foci. Similar results were obtained when FLAG-tagged WRN was used instead of GFP-WRN (data not shown). The effect of TSA could also be observed on the endogenous WRN protein. Incubation of WI-38 cells with TSA for 30 h led to a substantial increase in the number of cells in which WRN was excluded from the nucleoli with concurrent accumulation in nucleoplasmic foci (Fig. 5 C). Collectively, the data imply that the subcellular localization of WRN is regulated at least in part by protein acetylation. Furthermore, acetylation appears to play a role both in the exit of WRN from the nucleolus and in its subsequent return to the nucleolus, probably after the DNA damage response has subsided. In the absence of acetylation, WRN presumably retains its baseline nucleolar residence. The simplest way to account for these observations is by assuming that the regulatory acetylation events occur on the WRN protein itself. It was therefore important to determine whether WRN is subject to acetylation in living cells. To that end, 293T cells were transiently transfected with WRN expression plasmids, either alone or in combination with plasmids encoding the protein acetyltransferase, p300. Acetylated proteins were radiolabeled in vivo by incubation of the cultures with [3H]sodium acetate under conditions that are expected to restrict protein labeling largely to direct acetylation only. As shown in Fig. 6 A, lane 2, the immunoprecipitated WRN protein was specifically labeled in the presence of [3H]acetate, implying that it is indeed acetylatedin vivo. Cotransfection of WRN with p300 significantly increases WRN protein levels, presumably because of increased transcription from the transfected plasmids (data not shown). On the basis of previous calibration experiments, larger amounts of extract were therefore taken from cells transfected with WRN only. This indeed resulted in comparable amounts of total WRN protein in the different samples, as detected by Coomassie Blue staining (Fig. 6 B). As seen in Fig. 6 A, cotransfection with p300 substantially increased the extent of WRN acetylation (lane 3). These data imply that WRN is acetylated in vivo and suggest that p300 can contribute to this acetylation. If acetylation of WRN plays an important role in facilitating its exit from the nucleolus into nucleoplasmic foci, it might be predicted that p300, which augments this acetylation in vivo, will also increase WRN translocation. This prediction was tested in WRN-transfected U2OS cells. As expected, TSA treatment led to a marked enhancement of the translocation of GFP-WRN from the nucleolus into large nucleoplasmic foci (Fig. 7,WRN+ TSA). Importantly, cotransfection with p300 also resulted in a significant increase in the number of cells where GFP-WRN was present in such foci (WRN+p300); moreover, the microscopic pattern of these foci was indistinguishable from that elicited by TSA treatment (data not shown). Significantly, there was no apparent additive effect of p300 cotransfection and TSA treatment, supporting the notion that both agents were acting to increase WRN translocation via the same molecular mechanism, most probably acetylation. PML nuclear bodies (38Ruggero D. Wang Z.G. Pandolfi P.P. Bioessays. 2000; 22: 827-835Crossref PubMed Scopus (52) Google Scholar) constitute one well studied type of nucleoplasmic foci. It was of interest to find out whether any of the foci to which WRN relocated in irradiated cells represented PML bodies, particularly because the interaction of the PML protein with the cAMP response element-binding protein binding-protein (CBP)/p300 acetyltransferase (39Doucas V. Tini M. Egan D.A. Evans R.M. Proc. Natl. Acad. Sci. U. S. A. 1999; 96: 2627-2632Crossref PubMed Scopus (154) Google Scholar) could potentially underlie the coupling between WRN translocation and protein acetylation. Along this line, PML was shown to increase the acetylation of p53 by acetyltransferases residing in PML-NBs (40Pearson M. Carbone R. Sebastiani C. Cioce M. Fagioli M. Saito S. Higashimoto Y. Appella E. Minucci S. Pandolfi P.P. Pelicci P.G. Nature. 2000; 406: 207-210Crossref PubMed Scopus (1113) Google Scholar). Therefore, U2OS cells were transfected with GFP-WRN, exposed to 4J/m2 UV, and subjected to immunofluorescence analysis by confocal microscopy, employing antibodies directed against PML. As seen in Fig. 8, panelsD–F, there was indeed a partial colocalization of the large nucleoplasmic GFP-WRN foci with PML bodies. A similar pattern of partial colocalization was also revealed in cells analyzed 2 h after exposure to 40J/m2 UV (data not shown). In contrast, PML did not colocalize with the many minute nucleoplasmic speckles harboring GFP-WRN at later times after exposure to 40J/m2UV. A pattern of partial colocalization of GFP-WRN with PML bodies was also observed in cells treated with TSA in the absence of UV (Fig. 9, D–F). The nature of the remainder of the large nucleoplasmic foci, which contain GFP-WRN but do not stain positive for PML, still remains to be determined.Figure 9Partial colocalization of WRN and PML in TSA-treated cells. U2OS cells were transiently transfected with a GFP-WRN expression plasmid. 24 h later the cells were incubated for 24 h in the presence of TSA (D–F) or Me2SO (A–C) as a control. Cell staining and image processing were as in Fig. 7.View Large Image Figure ViewerDownload Hi-res image Download (PPT) Proper WRN activity is required for the efficient maintenance of genomic integrity, as reflected by the augmented genetic instability of cells derived from WS patients and by the increased rate of cancer in these patients (2Goto M. Miller R.W. Ishikawa Y. Sugano H. Cancer Epidemiol. Biomarkers Prev. 1996; 5: 239-246PubMed Google Scholar). In the present study, we report that several types of DNA damage mobilize WRN and cause its translocation from the nucleolus into discrete nucleoplasmic foci in a manner apparently dependent on the extent of DNA damage. These observations support a role for WRN in the DNA damage response, at least in some types of DNA damage. It is noteworthy that translocation of WRN from the nucleolus into the nucleoplasm is also induced by other types of stress, such as serum starvation (41Suzuki T. Shiratori M. Furuichi Y. Matsumoto T. Oncogene. 2001; 20: 2551-2558Crossref PubMed Scopus (49) Google Scholar). It is conceivable that WRN plays a role in the initial recognition of DNA damage or in the opening of the DNA damage site. Such a possibility is consistent with the fact that the helicase activity of WRN does not require the presence of a DNA terminus and particularly with the observations that WRN interacts with bubble structures (42Mohaghegh P. Karow J.K. Brosh R.M., Jr. Bohr V.A. Hickson I.D. Nucleic Acids Res. 2001; 29: 2843-2849Crossref PubMed Scopus (476) Google Scholar), which are good substrates for its exonuclease activity (10Shen J.C. Loeb L.A. Nucleic Acids Res. 2000; 28: 3260-3268Crossref PubMed Scopus (80) Google Scholar). Alternatively, WRN may play a role in later stages of the DNA repair process. For instance, WRN may act as a proofreading factor of the repair polymerase that seals up the DNA after removal of the initial damage-induced lesion. In line with such an idea, WRN has recently been shown to interact with the p50 subunit of DNA polymerase δ (Pol δ) and to stimulate the polymerization activity of Pol δ in the absence of PCNA (20Szekely A.M. Chen Y.H. Zhang C. Oshima J. Weissman S.M. Proc. Natl. Acad. Sci. U. S. A. 2000; 97: 11365-11370Crossref PubMed Scopus (102) Google Scholar) (43Kamath-Loeb A.S. Johansson E. Burgers P.M. Loeb L.A. Proc. Natl. Acad. Sci. U. S. A. 2000; 97: 4603-4608Crossref PubMed Scopus (156) Google Scholar). The ability of WRN to act as a proofreading factor for Pol δ may rely on its helicase and exonuclease activities (8Gray M.D. Shen J.C. Kamath-Loeb A.S. Blank A. Sopher B.L. Martin G.M. Oshima J. Loeb L.A. Nat. Genet. 1997; 17: 100-103Crossref PubMed Scopus (515) Google Scholar, 9Huang S., Li, B. Gray M.D. Oshima J. Mian I.S. Campisi J. Nat. 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This suggests that if WRN indeed plays a role in the DNA repair process, its activity is not obligatory and is not rate-limiting for the process, at least in the case of UV damage, but rather may be important for ensuring the quality of the repair. An alternative possibility is that WRN is involved in the signaling events that follow the occurrence of DNA damage. This is consistent with the finding that proper WRN function is required for efficient induction of p53 by UV and several other types of genotoxic agents (46Blander G. Zalle N. Leal J.F. Bar-Or R.L., Yu, C.E. Oren M. Faseb J. 2000; 14: 2138-2140Crossref PubMed Scopus (35) Google Scholar). For instance, WRN may transmit signals to upstream checkpoint control proteins, such as the CHK2 or ATM kinases (47Frei C. Gasser S.M. J. Cell Sci. 2000; 113 (Pt 15): 2641-2646Crossref PubMed Google Scholar, 48Frei C. Gasser S.M. Genes Dev. 2000; 14: 81-96PubMed Google Scholar). The translocation of WRN from the nucleolus into the nucleoplasm may enable it to interact with such proteins and transmit the activating signal to them. In this scenario, it is proposed that nucleolar WRN may be in a “standby” state, at least as it pertains to the DNA damage response, and that its mobilization into nucleoplasmic foci reflects its recruitment into an active mode. While this paper was under preparation, Sakamoto et al. (49Sakamoto S. Nishikawa K. Heo S.J. Goto M. Furuichi Y. Shimamoto A. Genes Cells. 2001; 6: 421-430Crossref PubMed Scopus (152) Google Scholar) also reported that WRN translocates to nucleoplasmic foci in response to several types of DNA damage. The findings implied that WRN plays a role in the recombination repair of double strand DNA breaks and in the processing of stalled replication forks. The rapid kinetics with which WRN translocates to nucleoplasmic foci after exposure to UV (our study) suggests that WRN also has a role in the response to other types of DNA damage, subject to nucleotide excision repair. A novel aspect of the present study is the finding that exposure to TSA or excess activity of p300, both of which are known to increase the level of protein acetylation, can strongly enhance the recruitment of WRN to nucleoplasmic foci. Acetylation might either decrease the interaction of WRN with protein(s) that retain it in the nucleolus in the absence of genotoxic stress or enhance its interaction with proteins that anchor it to the nucleoplasmic foci; these two possibilities are not mutually exclusive. The regulatory acetylation events may occur on any of a number of proteins that control the intracellular localization of WRN. However, the findings that WRN is acetylated in living cells and the demonstration that p300, which increases WRN acetylation in vivo, also promotes its translocation from the nucleolus into nucleoplasmic foci, argue strongly in favor of the notion that at least some of the critical acetylation events occur on the WRN protein itself. It is of note that nucleoplasmic WRN colocalizes partially with PML bodies. Interestingly, WRN can be modified by covalent attachment of SUMO-1 (50Kawabe Y. Seki M. Seki T. Wang W.S. Imamura O. Furuichi Y. Saitoh H. Enomoto T. J. Biol. Chem. 2000; 275: 20963-20966Abstract Full Text Full Text PDF PubMed Scopus (86) Google Scholar) and colocalizes with SUMO-1 in the nucleus (51Suzuki H. Seki M. Kobayashi T. Kawabe Y. Kaneko H. Kondo N. Harata M. Mizuno S. Masuko T. Enomoto T. Biochem. Biophys. Res. Commun. 2001; 286: 322-327Crossref PubMed Scopus (15) Google Scholar). Modification of the PML protein by SUMO-1 is thought to target PML to nuclear bodies (38Ruggero D. Wang Z.G. Pandolfi P.P. Bioessays. 2000; 22: 827-835Crossref PubMed Scopus (52) Google Scholar), raising the possibility that sumoylation of WRN is also involved in its recruitment to nuclear foci, particularly those coinciding with PML bodies. It is noteworthy that BLM, another member of the RecQ family, has also been shown to associate with PML bodies (52Wang X.W. Tseng A. Ellis N.A. Spillare E.A. Linke S.P. Robles A.I. Seker H. Yang Q., Hu, P. Beresten S. Bemmels N.A. Garfield S. Harris C.C. J. Biol. Chem. 2001; 276: 32948-32955Abstract Full Text Full Text PDF PubMed Scopus (124) Google Scholar); in that case, p53 appears directly responsible for recruiting BLM to these bodies. Once recruited to PML bodies, WRN may be modified by acetyltransferases present within these nuclear bodies (40Pearson M. Carbone R. Sebastiani C. Cioce M. Fagioli M. Saito S. Higashimoto Y. Appella E. Minucci S. Pandolfi P.P. Pelicci P.G. Nature. 2000; 406: 207-210Crossref PubMed Scopus (1113) Google Scholar), including p300. Because p53 is also recruited to such PML bodies, at least under some circumstances (40Pearson M. Carbone R. Sebastiani C. Cioce M. Fagioli M. Saito S. Higashimoto Y. Appella E. Minucci S. Pandolfi P.P. Pelicci P.G. Nature. 2000; 406: 207-210Crossref PubMed Scopus (1113) Google Scholar, 53Fogal V. Gostissa M. Sandy P. Zacchi P. Sternsdorf T. Jensen K. Pandolfi P.P. Will H. Schneider C. Del Sal G. EMBO J. 2000; 19: 6185-6195Crossref PubMed Scopus (317) Google Scholar, 54Ferbeyre G. de Stanchina E. Querido E. Baptiste N. Prives C. Lowe S.W. Genes Dev. 2000; 14: 2015-2027PubMed Google Scholar), these sites may promote the interaction between WRN and p53 (17Spillare E.A. Robles A.I. Wang X.W. Shen J.C., Yu, C.E. Schellenberg G.D. Harris C.C. Genes Dev. 1999; 13: 1355-1360Crossref PubMed Scopus (153) Google Scholar, 18Blander G. Kipnis J. Leal J.F., Yu, C.E. Schellenberg G.D. Oren M. J. Biol. Chem. 1999; 274: 29463-29469Abstract Full Text Full Text PDF PubMed Scopus (152) Google Scholar) and enable the WRN-dependent activation of p53 in response to UV and other types of genotoxic agents (46Blander G. Zalle N. Leal J.F. Bar-Or R.L., Yu, C.E. Oren M. Faseb J. 2000; 14: 2138-2140Crossref PubMed Scopus (35) Google Scholar). We thank Drs. S. Weissman, A. Dejean, and T. Mori for the gift of the GFP-WRN expression plasmid and for antibodies against PML, cyclobutane pyrimidine dimer (CPD), and 6–4PP, respectively.
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