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Multiple Forms of Immunoreactive β-Endorphin Are Present in an Ectopic Adrenocorticotropin-Producing Tumor but Not in Normal Pituitary or Pituitary Adenomas*

1982; Oxford University Press; Volume: 54; Issue: 1 Linguagem: Inglês

10.1210/jcem-54-1-167

1945-7197

Toshihiro Suda, FUMIKO TOZAWA, Hajime Yamaguchi, Tamotsu Shibasaki, Hiroshi Demura, KAZUO SHIZUME,

Neuropeptides and Animal Physiology

ABSTRACT. Human ACTH-producing tumor and plasma have been examined by gel filtration and ion exchange chromatography to detect the possible presence of reported multiple forms of immunoreactive β-endorphin (I-EP). Ion exchange chromatography of I-EP obtained from gel filtration showed four components of I-EP [two major peaks in the positions of EP-(1-31) and EP-(l-27) and two minor peaks in the positions of N-acetyl EP-(1-31) and iV-acetyl EP-(l-27)] in two ectopic ACTH-producing lung cancers, and two components of I-EP [the major peak in the position of EP-(1-31) and the minor peak i n the position of iV-acetyl EP-(1-31)] in an ectopic ACTH-producing thyroid cancer. Only a single peak in the position of EP-0-31) was present in plasma from a patient with Nelson’s syndrome and a patient with Addison’s disease, in the pituitary adenomas from six patients with Cushing’s disease, and in the nontumorous pituitary tissues from a patient with Cushing’s disease and a patient with acromegaly. These data suggest that the posttranslational processing of EP in human pituitary is different from that in the ectopic ACTH-producing tumor.