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Establishment of a New Extrahepatic Bile Duct Carcinoma Cell Line, TFK-1.

1995; Tohoku University; Volume: 177; Issue: 1 Linguagem: Inglês

10.1620/tjem.177.61

1349-3329

Susumu Saijyo, Toshio Kudo, Masanori Suzuki, Yu Katayose, Masao Shinoda, T Muto, Kenji Fukuhara, Takao Suzuki, Seiki Matsuno,

Colorectal Cancer Treatments and Studies

A new human extrahepatic bile duct carcinoma cell line (TFK-1) was established from a surgically resected tumor specimen, which was histologically diagnosed as partly papillary adenocarcinoma and partly differentiated tubular adenocarcinoma. The tumor cells cultured in RPMI-1640 medium supplemented with 10% FBS grew as monolayers showing epithelial-like morphology with a population doubling time of 37 hr during exponential growth at passage 40. Chromosome number was distributed in the range of 72 to 76, with a modal number of 73. Tumor markers (CEA, CA19-9, ST-439, DUPAN-2) were negative in culture supernatant and plasma of SCID mice grafted with TFK-1 cells. Though no point mutation at 12 codon of K-ras was detected, expression of c-erb B-2 product and MUC1 antigen was positive. TFK-1 is the third cell line established from extrahepatic bile duct carcinomas in the world literature, and should provide useful information on various aspects of this type of neoplasm.