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Three main patterns in the expression of six actin genes in the plerocercoid and adult Diphyllobothrium dendriticum tapeworm (Cestoda)

1997; Elsevier BV; Volume: 86; Issue: 2 Linguagem: Inglês

10.1016/s0166-6851(97)00034-0

1872-9428

Monica H. Wahlberg,

Insect Resistance and Genetics

The expression of six actin genes was examined in adult and plerocercoid Diphyllobothrium dendriticum tapeworms using in situ hybridization. On the basis of their structures, these genes are divided into three groups, the cestoda-I, -II and -III actins. Current studies show that the expression of actins belonging to different groups vary to a great extent. The three cestoda-I actins are expressed primarily in muscle cells of both adult and plerocercoid tapeworms, the expression being restricted to fewer cells in the plerocercoid larva. The two cestoda-II actins are cytoplasmic actin isoforms, expressed in a variety of cells, i.e. in cells dividing, differentiating and migrating. Expression of the cestoda-III actin gene is detected merely in the peripheral part of the outer parenchyma, mainly in the tegument cell bodies. This pattern is very weak in plerocercoids. The results indicate that actins also in D. dendriticum can be divided into cytoplasmic and muscle-specific isoforms. In this organism, one major pattern of muscle actin gene expression (cestoda-I) and two major patterns of non-muscle actin gene expression (cestoda-II and -III) were found.