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Site-specific recombinases: tools for genome engineering

1993; Elsevier BV; Volume: 9; Issue: 12 Linguagem: Inglês

10.1016/0168-9525(93)90104-p

1362-4555

Nigel J. Kilby, Michael Snaith, J. A. H. Murray,

Genomics and Chromatin Dynamics

Site-specific recombinases from bacteriophage and yeasts have been developed as novel tools for manipulating DNA both in the test-tube and in living organisms. We discuss the characteristics of these enzyme systems, review their application in genetic and developmental studies and speculate on their future potential for large-scale directed modifications of eukaryotic genomes.