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Circulating hemocytes from larvae of Melipona scutellaris (Hymenoptera, Apidae, Meliponini): Cell types and their role in phagocytosis

2009; Elsevier BV; Volume: 41; Issue: 2 Linguagem: Inglês

10.1016/j.micron.2009.10.003

1878-4291

Isabel Marques Rodrigues Amaral, João Felipe Moreira Neto, Gustavo Borges Pereira, Mariani Borges Franco, Marcelo Emı́lio Beletti, Warwick Estevam Kerr, Ana Maria Bonetti, Carlos Ueira‐Vieira,

Neurobiology and Insect Physiology Research

Infection in insects stimulates a complex defensive response. Recognition of pathogens may be accomplished by plasma or hemocyte proteins that bind specifically to bacterial or fungal polysaccharides. Several morphologically distinct hemocyte cell types cooperate in the immune response. Hemocytes attach to invading organisms and then isolate them by phagocytosis, by trapping them in hemocyte aggregates called nodules, or by forming an organized multicellular capsule around large parasites. In the current investigation the cellular in the hemolymph third instar larvae of M. scutellaris has been characterized by means of light microscopy analysis and phagocytosis assays were performed in vivo by injection of 0.5 μm fluorescence beads in order to identify the hemocyte types involved in phagocytosis. Four morphotypes of circulating hemocytes were found in 3rd instar larvae: prohemocytes, plasmatocytes, granulocytes and oenocytoids. The results presented plasmatocytes and granulocytes involved in phagocytic response of foreign particles in 3rd instar larvae of M. scutellaris.