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Simultaneous measurements of Ca 2+ in the intracellular stores and the cytosol of hepatocytes during hormone‐induced Ca 2+ oscillations

1995; Wiley; Volume: 368; Issue: 1 Linguagem: Inglês

10.1016/0014-5793(95)00632-j

1873-3468

Jean‐Yves Chatton, Haiyan Liu, Jörg W. Stucki,

Protein Kinase Regulation and GTPase Signaling

Simultaneous Ca 2+ measurements in the cytosol and intracellular stores (IS) of rat hepatocytes were performed using two Ca 2+ ‐sensitive probes (Fluo‐3 and Mag‐fura‐2), and combined whole‐cell patch clamp and fluorescence microscopy. A steady‐state Ca 2+ concentration of ∼630 μM was estimated in the IS. α 1 ‐Adrenergic stimulation induced periodic elevations of cytosolic Ca 2+ and parallel synchronized transient declines in the IS. Subsequent application of the intracellular Ca 2+ ‐pump inhibitor thapsigargin resulted in a release of Ca 2+ from the IS to reach a level of Ca 2+ depletion much lower than the lowest transient decline observed during the oscillations.