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BIBTEX RIS

Partial characterization of lipases produced by a newly isolated Penicillium sp. in solid state and submerged fermentation: A comparative study

2009; Elsevier BV; Volume: 42; Issue: 9 Linguagem: Inglês

10.1016/j.lwt.2009.04.006

1096-1127

Elisangela Wolski, Elisangele Menusi, Daniela Remonatto, Renata Vardanega, Francieli Arbter, Elisandra Rigo, Jorge Luiz Ninow, Márcio A. Mazutti, Marco Di Luccio, Débora de Olíveira, Helen Treichel,

Enzyme Production and Characterization

The aim of this research was the partial characterization of enzymatic extracts produced by a newly isolated Penicillium sp. in submerged (SmF) and solid state fermentation (SSF). The partial characterization of the crude enzymatic extract obtained by SSF and SmF systems showed optimum activity at pH 5.5 and 47 °C, and pH 7.0 and 37 °C, respectively (15.17 U/mL and 11.28 U/mL). The crude enzymatic extracts obtained by SmF and SSF presented the best stability at pH from 4.9 to 8.5 and temperature from 25 °C to 35 °C and pH 7.0 and 25 °C, respectively. These results confirm the interesting potential of SSF, because, besides the higher activities obtained in this system, the half-life time at 25 °C was higher than that observed for the lipase extract obtained in the SmF system.