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Comparison of measured oxyhemoglobin saturation and oxygen content with analyzer-calculated values and hand-calculated values obtained in unsedated healthy dogs

2005; American Veterinary Medical Association; Volume: 66; Issue: 7 Linguagem: Inglês

10.2460/ajvr.2005.66.1273

1943-5681

Nancy E. Scott, Steve C. Haskins, Janet Aldrich, Marlis L. Rezende, Ryan Gallagher, Matthew M. Henderson,

Cardiac Arrest and Resuscitation

Abstract Objective —To compare direct measurements of canine oxyhemoglobin (HbO 2 ) saturation and blood oxygen content (ContO 2 ) in healthy dogs with analyzer-calculated values derived by use of a human HbO 2 relationship and with hand-calculated values derived by use of a canine HbO 2 relationship. Animals —17 healthy dogs. Procedure —3-mL samples of heparinized arterial and jugular venous blood were collected from each dog. The pH, PCO 2 , PO 2 , hemoglobin, HbO 2 , carboxyhemoglobin, methemoglobin, and ContO 2 were measured; HbO 2 and ContO 2 were calculated automatically by analyzers and also hand-calculated. Blood gas analyzer–calculated and hand-calculated HbO 2 values were compared with co-oximeter–measured HbO 2 values. Analyzer-calculated and hand-calculated ContO 2 values were compared with oxygen content analyzermeasured values. Results —Hand-calculated HbO 2 values for arterial and jugular venous samples were slightly but significantly lower than those calculated by a blood gas analyzer or obtained from a co-oximeter. Hand-calculated and analyzer-calculated arterial and venous ContO 2 were similar to measured values. Conclusions and Clinical Relevance —Although certain HbO 2 and ContO 2 values generated by use of the different methods were significantly different, these differences are unlikely to be clinically important in healthy dogs. ( Am J Vet Res 2005;66:1273–1277)