Effects of sample preparation on matrix-assisted laser desorption/ionization time-of-flight mass spectra for sodium polystyrene sulfonate
1999; Wiley; Volume: 13; Issue: 11 Linguagem: Inglês
10.1002/(sici)1097-0231(19990615)13
ISSN1097-0231
AutoresRyuichi Arakawa, Shinji Watanabe, Tsuyoshi Fukuo,
Tópico(s)Analytical Chemistry and Chromatography
ResumoRapid Communications in Mass SpectrometryVolume 13, Issue 11 p. 1059-1062 Research Article Effects of sample preparation on matrix-assisted laser desorption/ionization time-of-flight mass spectra for sodium polystyrene sulfonate Ryuichi Arakawa, Corresponding Author Ryuichi Arakawa [email protected] Department of Applied Chemistry, Kansai University, Osaka, 564-8680 JapanDepartment of Applied Chemistry, Kansai University, Osaka, 564-8680 JapanSearch for more papers by this authorShinji Watanabe, Shinji Watanabe Department of Applied Chemistry, Kansai University, Osaka, 564-8680 JapanSearch for more papers by this authorTsuyoshi Fukuo, Tsuyoshi Fukuo Department of Applied Chemistry, Kansai University, Osaka, 564-8680 JapanSearch for more papers by this author Ryuichi Arakawa, Corresponding Author Ryuichi Arakawa [email protected] Department of Applied Chemistry, Kansai University, Osaka, 564-8680 JapanDepartment of Applied Chemistry, Kansai University, Osaka, 564-8680 JapanSearch for more papers by this authorShinji Watanabe, Shinji Watanabe Department of Applied Chemistry, Kansai University, Osaka, 564-8680 JapanSearch for more papers by this authorTsuyoshi Fukuo, Tsuyoshi Fukuo Department of Applied Chemistry, Kansai University, Osaka, 564-8680 JapanSearch for more papers by this author First published: 09 June 1999 https://doi.org/10.1002/(SICI)1097-0231(19990615)13:11 3.0.CO;2-1Citations: 16AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Studies have been made on the influence of matrix concentration and the selection of matrix-solvent combinations for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). Sodium polystyrene sulfonate (SPS) with five different degrees of polymerization, Mw = 1640, 4950, 7950, 16 600 and 57 500, were studied. The matrix 2′,3′,4′-trihydroxyacetophenone (THAP) was selected and was combined with acetonitrile, methanol, 2-propanol or water as the solvent. The MALDI-TOF experiments in negative ion mode reveal the following observations: (1) The concentration of the matrix must be increased as the degree of the polymerization increases; (2) depending on the degree of polymerization, there are different optimum combinations between the matrix and the polymer samples, and (3) different polarity of the solvents used in the matrix (THAP) preparation result in different MALDI spectra. Copyright © 1999 John Wiley & Sons, Ltd. REFERENCES 1M.Karas, U.Bahr, A.Deppe, B.Stahl and F.Hillenkamp, Macromol. Chem., Macromol. Symp. 61, 397 (1992); U.Bahr, A.Deppe, M.Karas, F.Hillenkamp and U.Giessmann, Macromol. Chem., Macromol. Symp. 64, 2866 (1992). 2P. O.Danis, D. E.Karr, F.Mayer, A.Holle and C. H.Watson, Org. Mass Spectrom. 27, 843 (1992); P. O.Danis, D. E.Karr, D. G.Westmoreland, M. C.Piton, D. I.Christie, P. A.Clay, S. H.Kable and R. G.Gilbert, Org. Mass Spectrom. 26, 6684 (1993). 3G.Montaudo, M. S.Montaudo, C.Puglisi and F.Samperi, Rapid Commun. Mass Spectrom. 9, 453 (1995). 4C.Jackson, B.Larsen and C.McEwen, Anal. Chem. 68, 1303 (1996). 5H.Rashidzadeh and B.Guo, Anal. Chem. 70, 131 (1998).Medline 6A. M.Belu, J. M.DeSimone, R. W.Linton, G. W.Lange and R. M.Friedman, J. Am. Soc. Mass Spectrom. 7, 11 (1996). 7A. T.Jackson, H. T.Yates, W. A.MacDonald, J. H.Scrivens, G.Gritchley, J.Brown, M. J.Deery, K. R.Jennings and C. M.Brookes, J. Am. Soc. Mass Spectrom. 8, 132 (1997). 8H. M. D.Liu and U. P.Schlunegger, Rapid Commun. Mass. Spectrom. 10, 483 (1996). 9G.Montaudo, M. S.Montaudo, C.Puglisi and F.Samperi, Rapid Commun. Mass Spectrom. 9, 1158 (1995). 10J. C.Blais, M.Tessier, G.Bolbach, B.Remaud, L.Rozes, J.Guittard, A.Burnot, E.Marechal and J. C.Tabet, Int. J. Mass Spectrom. Ion Processes 144, 131 (1995). 11A. K.Chaudhary, G.Critchley, A.Diaf and E. J.Beckmann, Macromolecules 29, 2213 (1996). 12J. B.Williams, A. I.Gusev and D. M.Hercules, Macromolecules 30, 3781 (1997). 13H. J.Rader and W.Schrepp, Acta Polymer 49, 272 (1998). and references cited therein. 14C. N.McEwen, C.Jackson and B. S.Larsen, Int. J. Mass Spectrom. Ion Processes 160, 387 (1997). 15H.Chen and B.Guo, Anal. Chem. 69, 4399 (1997).Medline 16H.Rashidzadeh and B.Guo, Anal. Chem. 70, 131 (1998).Medline 17N.Sakurada, T.Fukuo, K.Ute, K.Hatada and R.Arakawa, Rapid Commun. Mass. Spectrom. 12, 1895 (1998). 18D.Garrozzo, G.Impallomeni, E.Spina, L.Sturiale and F.Zanetti, Rapid Commun. Mass. Spectrom. 9, 937 (1995). 19G.Montaudo, D.Garrozzo, M. S.Montaudo, C.Puglisi and F.Samperi, Macromolecules 28, 7983 (1995). 20K. O.Borensen, M. A. S.Gass, G. J. M.Buruin, J. H. M.von Adruchem, M. C.Biro, G. M.Kresbach and M.Ehrat, Rapid Commum. Mass Spectrom. 11, 603 (1997). 21P. O.Danis and D. E.Karr, Macromolecules 28, 8548 (1995). 22H. J.Rader, J.Spickermann and K.Mullen, Macromol. Chem. Phys. 196, 3967 (1995). 23W. W.Yau, J. J.Kirkland and D. D.Bly, Modern Size-Exclusion Liquid Chromatography; John Wiley and Sons, New York (1979). Citing Literature Volume13, Issue1115 June 1999Pages 1059-1062 ReferencesRelatedInformation
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