Synthesis of polypeptide models of elastin
1983; Wiley; Volume: 21; Issue: 4 Linguagem: Inglês
10.1111/j.1399-3011.1983.tb03115.x
ISSN0367-8377
AutoresRao S. Rapaka, Kouji Okamoto, Marianna M. Long, Dan W. Urry,
Tópico(s)Elasticity and Material Modeling
ResumoSynthesis of two copolymers, H‐(φO‐Pro‐Gly‐Gly) n ‐Val‐OMe and H‐(φO‐Pro‐Gly‐Gly) n ‐Val‐OMe, where φO is Val or Lys and φO is Val or Glu is described. Cross‐linking between the two copolymers is achieved by a coupling reaction between the εe‐amino groups of the lysine containing copolymer and δ‐carboxyls of the glutamic acid of the other copolymer. The cross‐linking reaction was performed during a temperature elicited phase separation with flow orientation of the copolymers. Coacervation of the intermediate polymers is presented, as is the scanning electron micrograph of the insoluble cross‐linked product and its calcifiability as determined with electron probe microanalysis. The purity of the key intermediates and polymers is demonstrated by the usual analytical methods. Carbon‐13 magnetic resonance spectra of the intermediate monomer units are included to validate the purity of their synthesis.
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