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Association of neutral deoxyribonuclease with chromatin isolated from mammalian cells

1967; Elsevier BV; Volume: 149; Issue: 2 Linguagem: Inglês

10.1016/0005-2787(67)90174-8

1879-3002

Karl F. Swingle, Leonard J. Cole, Julie Bailey,

RNA Interference and Gene Delivery

Nuclei prepared from spleen cells by a variety of published procedures retained a deoxyribonuclease which caused a partial degradation of the nuclear DNA when the nuclei were incubated in the presence of divalent cations. Well-washed chromatin prepared according to Paul and Gilmour7 from disrupted nuclei of either spleen or liver retained the deoxyribonuclease, but the soluble nucleoprotein of Zubay and Doty5 was free of it. The latter preparation was also resistant to attack by added deoxyribonuclease I. The nuclear deoxyribonuclease was shown to be of the deoxyribonuclease I type, which produces 3′-hydroxyl-terminated fragments of DNA. X-Irradiation of washed nuclei in vitro did not alter their susceptibility to degradation by their endogenous deoxyribonuclease. Chromatin from ‘free’ thymocytes did not contain endogenous deoxyribonuclease I activity.